Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial
Effects of chemical form of selenium on plasma biomarkers in a high-dose human supplementation trial
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DOI：
10.1158/1055-9965.epi-05-0950
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发表时间：
2006-04-01
影响因子：
3.8
通讯作者：
Byrne, DW
中科院分区：
文献类型：
--
作者：
Burk, RF;Norsworthy, BK;Byrne, DW
目前正在进行不同形式硒的干预试验，以评估硒补充剂对癌症和其他疾病发病率的影响。血浆硒生物标志物对硒给药有反应，可能有助于评估这些试验的依从性和安全性。本研究旨在探讨补硒对高硒人群血浆硒生物标志物和尿硒排泄的影响。给予亚硒酸钠、高硒酵母（酵母）和L-硒代甲硫氨酸（硒代甲硫氨酸）形式的中量（类似于200 μ g/d）至大量（类似于600 μ g/d）硒补充剂。受试者被随机分为10组（安慰剂和每种形式硒的三个剂量水平）。血浆生物标志物（硒浓度，硒蛋白P浓度和谷胱甘肽过氧化物酶活性）在补充前和每4周测定一次，共16周。在16周时测定尿硒排泄量。补充硒蛋氨酸和酵母以剂量依赖的方式提高血浆硒浓度。Selenite没有。硒浓度的增加与硒代蛋氨酸的施用量相关。谷胱甘肽过氧化物酶活性和硒蛋白P浓度对补硒均无反应。尿硒排泄后硒蛋氨酸大于亚硒酸盐后，与酵母后的中间和其他两个没有显着差异的排泄。我们的结论是，血浆硒浓度是有用的监测遵守和安全的硒补充硒蛋氨酸，而不是亚硒酸盐。血浆硒似乎反映了酵母的硒蛋氨酸含量，但不是其他形式的酵母硒。根据尿硒排泄量判断，硒以硒代甲硫氨酸的形式比亚硒酸盐更好地被吸收。
Intervention trials with different forms of selenium are under way to assess the effects of selenium supplements on the incidence of cancer and other diseases. Plasma selenium biomarkers respond to selenium administration and might be useful for assessing compliance and safety in these trials. The present study characterized the effects of selenium supplementation on plasma selenium biomarkers and urinary selenium excretion in selenium-replete subjects. Moderate (similar to 200 mu g/d) to large (similar to 600 mu g/d) selenium supplements in the forms sodium selenite, high-selenium yeast (yeast), and L-selenomethionine (selenomethionine) were administered. Subjects were randomized into 10 groups (placebo and three dose levels of each form of selenium). Plasma biomarkers (selenium concentration, selenoprotein P concentration, and glutathione peroxidase activity) were determined before supplementation and every 4 weeks for 16 weeks. Urinary selenium excretion was determined at 16 weeks. Supplementation with selenomethionine and yeast raised the plasma selenium concentration in a dose-dependent manner. Selenite did not. The increased selenium concentration correlated with the amount of selenomethionine administered. Neither glutathione peroxidase activity nor selenoprotein P concentration responded to selenium supplementation. Urinary selenium excretion was greater after selenomethionine than after selenite, with excretion after yeast being intermediate and not significantly different from either of the other two. We conclude that plasma selenium concentration is useful in monitoring compliance and safety of selenium supplementation as selenomethionine but not as selenite. Plasma selenium seems to reflect the selenomethionine content of yeast but not the other yeast selenium forms. As judged by urinary selenium excretion, selenium in the form of selenomethionine is better absorbed than selenite.