Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal
Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal
复制标题
原子力显微镜和先进激光共焦显微镜联合操作观察蛋白质晶体的表面过程
DOI:
10.4236/jsemat.2012.223032
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发表时间:
2012
期刊:
影响因子:
--
通讯作者:
N. Goto
中科院分区:
文献类型:
--
作者:
S. Yanagiya;N. Goto
We demonstrated the insitu observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). The AFM cantilever scanned or indented the {110} surface of a hen egg-white lysozyme crystal in a supersaturated solution. Using a soft cantilever, we could observe the step growth with high time resolution by LCM-DIM and perform quantitative measurements of the step height by AFM simultaneously. In addition, a hard cantilever was used with LCM-DIM to observe the dynamics of crystal surface scratching and indentation. In the supersaturated solution, the small steps generated from the scratched line aggregated to macro steps, and subsequently flattened the surface.
影响因子:
3.8
作者:
Van Driessehe Alexander Es;G. Sazaki;戴国亮;F. Otálora;Gavira Ja;T. Matsui;I. Yoshizaki;K. Tsukamoto;K. Nakajima
通讯作者:
Van Driessehe Alexander Es;G. Sazaki;戴国亮;F. Otálora;Gavira Ja;T. Matsui;I. Yoshizaki;K. Tsukamoto;K. Nakajima
DOI:
--
发表时间:
2004
期刊:
J.Crystal Growth 262
影响因子:
--
作者:
G.Sazaki;et al.
通讯作者:
et al.