Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal

Joint Operation of Atomic Force Microscope and Advanced Laser Confocal Microscope for Observing Surface Processes in a Protein Crystal
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原子力显微镜和先进激光共焦显微镜联合操作观察蛋白质晶体的表面过程

DOI:
10.4236/jsemat.2012.223032
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发表时间:
2012
期刊:
Journal of Surface Engineered Materials and Advanced Technology
影响因子:
--
通讯作者:
N. Goto
N. Goto
中科院分区:
--
文献类型:
--
作者:
S. Yanagiya;N. Goto

文献摘要

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我们演示了使用激光共焦显微镜与微分干涉显微镜 (LCM-DIM) 相结合对移动原子力显微镜 (AFM) 悬臂进行原位观察。 AFM 悬臂扫描或压痕过饱和溶液中鸡蛋清溶菌酶晶体的 {110} 表面。使用软悬臂梁,我们可以通过LCM-DIM以高时间分辨率观察台阶生长,并同时通过AFM对台阶高度进行定量测量。此外,硬悬臂与LCM-DIM一起使用来观察晶体表面划痕和压痕的动态。在过饱和溶液中,从划痕线产生的小台阶聚集成大台阶,随后使表面变平。
We demonstrated the insitu observation of a moving atomic force microscope (AFM) cantilever using a laser confocal microscope combined with a differential interference microscope (LCM-DIM). The AFM cantilever scanned or indented the {110} surface of a hen egg-white lysozyme crystal in a supersaturated solution. Using a soft cantilever, we could observe the step growth with high time resolution by LCM-DIM and perform quantitative measurements of the step height by AFM simultaneously. In addition, a hard cantilever was used with LCM-DIM to observe the dynamics of crystal surface scratching and indentation. In the supersaturated solution, the small steps generated from the scratched line aggregated to macro steps, and subsequently flattened the surface.
DOI: 10.1021/cg8006684
发表时间: 2009-05
影响因子: 3.8
作者:
Van Driessehe Alexander Es;G. Sazaki;戴国亮;F. Otálora;Gavira Ja;T. Matsui;I. Yoshizaki;K. Tsukamoto;K. Nakajima
通讯作者: Van Driessehe Alexander Es;G. Sazaki;戴国亮;F. Otálora;Gavira Ja;T. Matsui;I. Yoshizaki;K. Tsukamoto;K. Nakajima
激光共聚焦显微镜原位观察蛋白质晶体表面的基本生长台阶
DOI: --
发表时间: 2004
期刊: J.Crystal Growth 262
影响因子: --
作者:
G.Sazaki;et al.
通讯作者: et al.