Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair
Nanofibrillar Decellularized Wharton's Jelly Matrix for Segmental Tracheal Repair
复制标题
用于分段气管修复的纳米原纤维脱细胞沃顿氏胶基质
DOI：
10.1002/adfm.201910067
复制
发表时间：
2020-02-19
影响因子：
19
通讯作者：
Yang, Yang
中科院分区：
文献类型：
--
作者：
Xu, Yong;Duan, Hang;Yang, Yang
沃顿氏胶（WJ）被认为是组织工程气管中潜在的支架，因为它的成分和功能与软骨组织相似。然而，利用WJ构建工程化新软骨组织的可行性尚未见报道，更遑论气管管状软骨再生和气管节段性病变修复。本文制备了由三种不同脱细胞WJ基质(DWJM)/聚（epsilon-己内酯）（PCL）比例（8:2,5:5和2:8）组成的电纺丝纳米纤维膜。结果表明，随着DWJM含量的增加，降解速度、吸收能力和细胞粘附能力提高，但力学性能减弱，但在体内培养12周后，DWJM/PCL（8:2）组才实现了令人满意的均匀软骨再生。此外，在二维纳米纤维膜的基础上，利用改进的三明治模型构建了具有可控管腔直径和壁厚的均匀三维管状气管状软骨，其中软骨细胞膜结构在硅管周围滚动。最重要的是，通过将上述方案与先前建立的血管化和上皮化技术相结合，同时实现软骨化、血管化和上皮化，从而在具有与天然气管相似生物结构和功能的兔模型中实现长期（6个月）的气管周长损伤修复，为气管组织工程的临床应用提供了一种很有前景的途径。
Wharton's jelly (WJ) is considered a potential scaffold in tissue-engineered trachea for its similar composition and function to cartilage tissue. However, the feasibility of using WJ to construct engineered neocartilage tissue has not been reported, let alone tubular tracheal cartilage regeneration and segmental tracheal lesion repair. Here, electrospun nanofibrous membranes composed of three different decellularized WJ matrix (DWJM)/poly(epsilon-caprolactone) (PCL) ratios (8:2, 5:5, and 2:8) are fabricated. The results demonstrate improved degradation speed, absorption, and cell adhesion capacity but weakened mechanical properties with increased DWJM content, but satisfactory homogeneous cartilage regeneration is only achieved in the DWJM/PCL (8:2) group after 12 weeks in vivo culture. Furthermore, homogeneous, 3D, tubular, trachea-shaped cartilage is constructed with a controllable lumen diameter and wall thickness based on the 2D nanofibrous membrane using a modified sandwich model, in which the chondrocyte-membrane construct is rolled around a silicon tube. Most importantly, by combining the above schemes with previously established vascularization and epithelialization techniques, chondrification, vascularization, and epithelialization are achieved simultaneously thus realizing long-term (6 months) circumferential tracheal lesion repair in a rabbit model with a biological structure and function similar to that of native trachea, representing a promising approach for the clinical application of tracheal tissue engineering.