Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography-Mass Spectrometry.
Quantitative Profiling of Lipid Species in Caenorhabditis elegans with Gas Chromatography-Mass Spectrometry.
复制标题
使用气相色谱-质谱法对秀丽隐杆线虫中的脂质种类进行定量分析。
DOI：
10.1007/978-1-0716-0592-9_10
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Soukas,AlexanderA
中科院分区：
文献类型：
--
作者：
Pino,ElizabethC;Soukas,AlexanderA
气相色谱-质谱法（GC-MS）能够灵敏地检测和相对定量脂肪酸。在秀丽隐杆线虫中，使用GC-MS可以证实常见染色方法的结果，提供了在衰老、遗传突变或饮食或药理学操作中大量改变的脂质种类的高分辨率。在这里，我们描述了一种方法来定量总蠕虫脂质提取物中脂肪酸的相对丰度，以及一种方法，定量脂肪酸分离成中性脂质池（三酰甘油和胆固醇酯）与极性更强的脂质（磷脂）的固相萃取（SPE）。
Gas chromatography–mass spectrometry (GC-MS) enables sensitive detection and relative quantification of fatty acids. InCaenorhabditis elegans, the use of GC-MS can corroborate findings from common staining methodologies, providing great resolution on the lipid species altered in abundance in aging, genetic mutants, or with dietary or pharmacologic manipulation. Here we describe a method to quantitate relative abundance of fatty acids in total worm lipid extracts, as well as a method that quantitates fatty acids following separation into neutral lipid pools (triacylglycerols and cholesteryl esters) versus more polar lipids (phospholipids) by solid-phase extraction (SPE).