Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin
Measurements of UV-generated free radicals/reactive oxygen species (ROS) in skin
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DOI：
10.1016/j.saa.2005.10.013
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发表时间：
2006-03-13
影响因子：
4.4
通讯作者：
Fuchs, J
中科院分区：
文献类型：
--
作者：
Herrling, T;Jung, K;Fuchs, J
用电子自旋共振（ESR）技术检测紫外线照射皮肤产生的自由基/活性氧（ROS）。为了提高测量的灵敏度，使用硝酰基探针3-羧基-2，2，5，5-四甲基吡咯烷-1-氧自由基（PCA）清除和积累短寿命自由基/活性氧。自由基/ROS的空间分布测量在猪皮肤活检与ESR成像UV照射后对应于在皮肤的深度的辐照度的强度衰减。自由基/ROS的主要部分由UVA（320-400 nm）产生，使得自由基的空间分布达到真皮的下侧。在人体皮肤上的活体测量是用L波段ESR光谱仪和表面线圈进行的，该表面线圈将来自所有皮肤层的信号强度进行积分以获得足够的信号幅度。使用该实验装置，测量UVB和UVA/B滤光片对皮肤中自由基/ROS产生的保护。对ROS的保护和由它们引起的损伤的修复可以通过以高抗氧化能力（AP）为特征的活性抗氧化剂来实现。紫外线过滤剂和抗氧化剂的效果对应于它们对皮肤中由UVAB照射产生的自由基/ROS的保护，可以通过新的自由基防晒因子（RSF）来量化。RSF表示停留在阳光下的时间增加，以在皮肤中产生与未受保护的皮肤相同数量的自由基/ROS。关于皮肤中产生的自由基/ROS的量作为生物物理终点，RSF表征了对UVB和UVA辐射的保护。(c)2005 Elsevier B. V.保留所有权利。
Free radicals/reactive oxygen species (ROS) generated in skin by UV irradiation were measured by electron spin resonance (ESR). To increase the sensitivity of measurement the short life free radicals/ROS were scavenged and accumulated by using the nitroxyl probe 3-carboxy-2,2,5,5-tetrametylpyrrolidine-1-oxyl (PCA). The spatial distribution of free radicals/ROS measured in pig skin biopsies with ESR imaging after UV irradiation corresponds to the intensity decay of irradiance in the depth of the skin. The main part of free radicals/ROS were generated by UVA (320-400 nm) so that the spatial distribution of free radicals reaches up to the lower side of the dermis. In vivo measurements on human skin were performed with a L-band ESR spectrometer and a surface coil integrating the signal intensities from all skin layers to get a sufficient signal amplitude. Using this experimental arrangement the protection of UVB and UVA/B filter against the generation of free radicals/ROS in skin were measured. The protection against ROS and the repair of damages caused by them can be realized with active antioxidants characterized by a high antioxidative power (AP). The effect of UV filter and antioxidants corresponding to their protection against free radicals/ROS in skin generated by UVAB irradiation can be quantified by the new radical sun protection factor (RSF). The RSF indicates the increase of time for staying in the sun to generate the same number of free radicals/ROS in the skin like for the unprotected skin. Regarding the amount of generated free radicals/ROS in skin as an biophysical endpoint the RSF characterizes both the protection against UVB and UVA radiation. (c) 2005 Elsevier B.V. All rights reserved.