The 5′ third of Cauliflower mosaic virus gene VI conditions resistance breakage in Arabidopsis ecotype tsu-0
The 5′ third of Cauliflower mosaic virus gene VI conditions resistance breakage in Arabidopsis ecotype tsu-0
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DOI：
10.1094/phyto.2002.92.2.190
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发表时间：
2002-02-01
期刊：
影响因子：
3.2
通讯作者：
Leisner, SM
中科院分区：
文献类型：
--
作者：
Agama, K;Beach, S;Leisner, SM
拟南芥生态型对病毒的反应各不相同。本研究分析了A.拟南芥生态型大津-0对花椰菜花叶病毒（CaMV）的抗性。该生态型先前报道对两个CaMV分离物（CM 1841和CM 4 -184）具有抗性，但对W260敏感。在这项研究中，我们表明，大津-0是耐四个额外的CaMV分离物。CaMV在大津-0植株的莲座叶内繁殖，但似乎没有系统地传播到花序中。然而，大津-0植物莲座叶中的病毒活力显然没有受到损害，因为感染性CaMV可以从这些器官中回收。W260通过被动机制（即，这种病毒避免激活植物防御）。负责W260抗性断裂的病毒基因组部分被定位到基因VI的5'三分之一，我们称之为RBR-1。该区域还负责控制CaMV感染不同类型的茄科植物的能力。因此，不同家族的植物与CaMV相互作用的途径可能通过进化而保守。
Arabidopsis thaliana ecotypes vary in their responses to viruses. In this study, we analyzed the variation in response of A. thaliana ecotype Tsu-0 to Cauliflower mosaic virus (CaMV). This ecotype was previously reported to be resistant to two CaMV isolates (CM1841 and CM4-184), but susceptible to W260. In this study, we show that Tsu-0 is resistant to four additional CaMV isolates. CaMV propagated within the rosette leaves of Tsu-0 plants, but did not appear to spread systemically into the in-florescence. However, virus viability in rosette leaves of Tsu-0 plants apparently was not compromised because infectious CaMV could be recovered from these organs. W260 overcomes Tsu-0 resistance by a passive mechanism (i.e., this virus avoids activating plant defenses). The portion of the viral genome responsible for W260 resistance breakage was mapped to the 5' third of gene VI, which we have termed RBR-1. This region is also responsible for controlling the ability of CaMV to infect different types of solanaceous plants. Hence, the pathways by which plants of different families interact with CaMV may be conserved through evolution.