A mitochondrial protein increases glycolytic flux
A mitochondrial protein increases glycolytic flux
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DOI：
10.1016/j.meatsci.2017.06.007
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发表时间：
2017-11-01
期刊：
影响因子：
7.1
通讯作者：
Gerrard, David E.
中科院分区：
文献类型：
--
作者：
Matarneh, Sulaiman K.;England, Eric M.;Gerrard, David E.
本研究的目的是确定线粒体在死后肌肉代谢中的作用。将分离的线粒体掺入反应缓冲液中，在有或没有线粒体电子传递链抑制剂的情况下模拟死后糖酵解。无论抑制剂如何，添加线粒体都会在 240 和 1440 分钟降低 pH 值。 pH值的降低伴随着糖原降解和乳酸积累的增强。为了探索造成这种过度代谢的机制，对线粒体制剂进行了机械破坏和离心。将所得上清液和沉淀分别添加至体外模型中。线粒体上清液产生与包含完整线粒体的上清液相似的效果。为了进一步缩小我们的研究目标，用高氯酸对线粒体上清液进行脱蛋白。高氯酸处理后线粒体上清液的作用消失。这些数据表明，基于线粒体的蛋白质能够在体外模型中增加糖酵解通量，并可能部分解释高度氧化的 AMPK gamma 3(R200Q) 突变猪中酸肉的发育。
The purpose of this study was to determine the role of mitochondria in postmortem muscle metabolism. Isolated mitochondria were incorporated into a reaction buffer that mimics postmortem glycolysis with or without mitochondrial electron transport chain inhibitors. Addition of mitochondria lowered pH values at 240 and 1440 min regardless of inhibitors. Reduction in pH was accompanied by enhanced glycogen degradation and lactate accumulation. To explore the mechanism responsible for this exaggerated metabolism, mitochondrial preparations were mechanically disrupted and centrifuged. Resulting supernatants and pellets each were added to the in vitro model. Mitochondrial supernatants produced similar effects as those including intact mitochondria. To narrow further our target of investigation, mitochondrial supernatants were deproteinized with perchloric acid. The effect of mitochondrial supernatant was lost after perchloric acid treatment. These data indicate that a mitochondrial-based protein is capable of increasing glycolytic flux in an in vitro model and may partially explain acid meat development in highly oxidative AMPK gamma 3(R200Q) mutated pigs.