GAMMA ACTIN, SPECTRIN, AND INTERMEDIATE FILAMENT PROTEINS COLOCALIZE WITH VINCULIN AT COSTAMERES, MYOFIBRIL-TO-SARCOLEMMA ATTACHMENT SITES
GAMMA ACTIN, SPECTRIN, AND INTERMEDIATE FILAMENT PROTEINS COLOCALIZE WITH VINCULIN AT COSTAMERES, MYOFIBRIL-TO-SARCOLEMMA ATTACHMENT SITES
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DOI：
10.1002/cm.970030513
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
PARDO, JV
中科院分区：
文献类型：
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作者：
CRAIG, SW;PARDO, JV
定位纽蛋白在横纹肌纤维肌膜定义了一个正交的晶格。网格的中粒是粘着斑蛋白的肋状条带，其垂直于纤维的长轴延伸，与肌下肌原纤维的I条带对齐地重复，并且似乎将肌原纤维偶联到肌膜[Pardo et al 1982，1983a]。在本文中提出的共定位研究表明，γ肌动蛋白，血影蛋白，和中间丝抗原是额外的组成部分，这种晶格的costameres。此外，结果表明，γ肌动蛋白和血影蛋白也是衣领的内部网络的组成部分，首先用结蛋白的抗体显现[格兰杰和拉扎里德斯，1978]，其在Z线水平将肌原纤维彼此连接。
Localization of vinculin at the sarcolemma of striated muscle fibers defines an orthogonal lattice. The costameres of the lattice are the riblike bands of vinculin that run perpendicular to the long axis of the fiber, repeat in register with I bands of the subjacent myofibrils, and seem to couple the myofibril to the sarcolemma [Pardo et al 1982, 1983a]. The colocalization studies presented in this paper show that gamma actin, spectrin, and intermediate filament antigens are additional components of this lattice of costameres. In addition, the results show that gamma actin and spectrin are also components of the internal network of collars, first visualized with antibody to desmin [Granger and Lazarides, 1978], that connects the myofibrils to each other at the level of the Z line.