GLUCOSE TRANSPORTERS SERVE AS WATER CHANNELS
GLUCOSE TRANSPORTERS SERVE AS WATER CHANNELS
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DOI：
10.1073/pnas.87.8.3244
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发表时间：
1990-04-01
影响因子：
11.1
通讯作者：
ROSEN, OM
中科院分区：
文献类型：
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作者：
FISCHBARG, J;KUANG, K;ROSEN, OM
水穿过一些真核细胞的质膜的速度比它们的脂质双层的透水性所能解释的要快。这导致了对水道的搜索。我们以前的工作确定葡萄糖转运体是这样一个通道的候选者。在此，我们报道了注射编码脑/Hep G2、成年骨骼肌/脂肪细胞或肝脏形式的葡萄糖转运蛋白的非洲爪哇卵母细胞，其质膜的渗透性比未处理的卵母细胞大。在注射的卵母细胞中，由葡萄糖转运蛋白引起的渗透水渗透性成分平均增加了4.8倍。这些研究提供了直接证据，证明哺乳动物的促进性、钠非依赖性葡萄糖转运体作为膜水通道发挥作用。
Water traverses the plasma membranes of some eukaryotic cells faster than can be explained by the water permeability of their lipid bilayers. This has led to a search for a water channel. Our previous work identified glucose transporters as candidates for such a channel. We report here that Xenopus laevis oocytes injected with mRNA encoding the brain/Hep G2, adult skeletal muscle/adipocyte, or liver forms of the glucose transporter exhibit an osmotic water permeability of their plasma membranes larger than that of untreated oocytes. The osmotic water permeability component attributable to glucose transporters increased an average of 4.8-fold in the injected oocytes. These studies provide direct evidence that the facilitative, sodium-independent mammalian glucose transporters serve as membrane water channels.