Narrow-Band Ultraviolet B Phototherapy Ameliorates Acute Graft-Versus-Host Disease of the Intestine by Expansion of Regulatory T Cells.
Narrow-Band Ultraviolet B Phototherapy Ameliorates Acute Graft-Versus-Host Disease of the Intestine by Expansion of Regulatory T Cells.
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DOI：
10.1371/journal.pone.0152823
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Kato J
中科院分区：
文献类型：
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作者：
Hashimoto A;Sato T;Iyama S;Yoshida M;Ibata S;Tatekoshi A;Kamihara Y;Horiguchi H;Murase K;Kawano Y;Takada K;Miyanishi K;Kobune M;Ichimiya S;Kato J
窄带紫外线B （NB-UVB）在皮肤光疗中有着广泛的应用。关于NB-UVB光疗在移植物抗宿主病（graft- anti -host disease， GVHD）中的应用，我们之前报道过它对急性移植物抗宿主病（aGVHD）的皮肤病变非常有效，NB-UVB诱导的调节性T细胞（Treg）扩增可能是其机制之一。为了检验通过扩增Treg细胞的NB-UVB照射是否不仅对皮肤aGVHD有效，而且对肠道或肝脏等内脏器官aGVHD也有效，我们进行了实验，用NB-UVB照射小鼠致死性aGVHD模型，其特征是严重累及肠道。我们发现NB-UVB照射可提高临床评分和生存率。aGVHD的病理评分在所有受累器官：肠、肝、皮肤均有改善。在NB-UVB照射小鼠血清中，Treg细胞相关细胞因子如转化生长因子β (TGFβ)和白细胞介素-10 （IL-10）水平升高。NB-UVB可增加小鼠肠道和脾脏炎症组织中Treg细胞的浸润数量。这是第一个证明NB-UVB光疗能够通过扩增Treg细胞来改善肠道aGVHD的报道。
Narrowband ultraviolet B (NB-UVB) has been widely used in dermatological phototherapy. As for the application of NB-UVB phototherapy to graft-versus-host disease (GVHD), we previously reported that it was highly efficacious for cutaneous lesions of acute GVHD (aGVHD) and that expansion of regulatory T (Treg) cells induced by NB-UVB might be one of the mechanisms. In order to examine whether NB-UVB irradiation through expansion of Treg cells is effective for the treatment of not only cutaneous aGVHD but also aGVHD of inner organs such as the intestine or liver, we conducted experiments in which a murine lethal aGVHD model, characterized by severe involvement of the intestine, was irradiated with NB-UVB. We found that NB-UVB irradiation improved the clinical score and survival rate. The pathological score of aGVHD was improved in all affected organs: intestine, liver, and skin. In the serum of mice irradiated with NB-UVB, the levels of Treg cells-associated cytokines such as transforming growth factor beta (TGFβ) and interleukin-10 (IL-10) were elevated. The numbers of infiltrating Treg cells in inflamed tissue of the intestine and those in spleen were increased in mice treated with NB-UVB. This is the first report demonstrating that NB-UVB phototherapy has the ability to ameliorate intestinal aGVHD through the expansion of Treg cells.