Interaction with PCNA is essential for yeast DNA polymerase η function
Interaction with PCNA is essential for yeast DNA polymerase η function
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DOI：
10.1016/s1097-2765(01)00319-7
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发表时间：
2001-08-01
期刊：
影响因子：
16
通讯作者：
Prakash, L
中科院分区：
文献类型：
--
作者：
Haracska, L;Kondratick, CM;Prakash, L
在酵母和人类中，DNA聚合酶(Pol)ETA在紫外线损伤的DNA的无错误复制中发挥作用，Pol ETA也通过许多其他DNA损伤促进复制。在这里，我们提出了酵母Pol ETA与增殖细胞核抗原(增殖细胞核抗原)的物理和功能相互作用的证据，并表明与增殖细胞核抗原的相互作用对于Pol ETA的体内功能是必不可少的。POL ETA在插入与基本位点相反的核苷酸时效率很低，但与增殖细胞核抗原的相互作用极大地刺激了其相对于该损伤的核苷酸掺入能力。因此，除了在Pol ETA靶向DNA损伤的复制机制方面发挥关键作用外，与增殖细胞核抗原的相互作用还将促进某些DNA损伤的旁路。
In both yeast and humans, DNA polymerase (Pol) eta functions in error-free replication of ultraviolet-damaged DNA, and Pol eta promotes replication through many other DNA lesions as well. Here, we present evidence for the physical and functional interaction of yeast Pol eta with proliferating cell nuclear antigen (PCNA) and show that the interaction with PCNA is essential for the in vivo function of Pol eta. Pol eta is highly inefficient at inserting a nucleotide opposite an abasic site, but interaction with PCNA greatly stimulates its ability for nucleotide incorporation opposite this lesion. Thus, in addition to having a pivotal role in the targeting of Pol eta to the replication machinery stalled at DNA lesions, interaction with PCNA would promote the bypass of certain DNA lesions.