Bioengineered olfactory sensory neuron-based biosensor for specific odorant detection
Bioengineered olfactory sensory neuron-based biosensor for specific odorant detection
复制标题
用于特定气味检测的生物工程嗅觉感觉神经元生物传感器
DOI：
10.1016/j.bios.2012.08.035
复制
发表时间：
2013-02-15
影响因子：
12.6
通讯作者：
Wang, Ping
中科院分区：
文献类型：
--
作者：
Du, Liping;Wu, Chunsheng;Wang, Ping
获得具有特异性嗅觉受体（ORs）的功能细胞用于开发基于细胞的气味检测生物传感器是非常期望的。在这项研究中，我们探讨了生物工程的初级嗅觉感觉神经元（OSNs）作为仿生嗅觉生物传感器的敏感元件的可行性，其中光寻址电位传感器（LAPS）用于监测生物工程OSNs的膜电位对气味分子的反应。C. elegances，ODR-10作为模型受体，通过瞬时转染在OSNs的质膜上表达。通过分析细胞外电位的频域和时域特征，研究了生物工程化OSNs对气味分子的响应特性。结果表明，生物工程OSNs可以特异性响应双乙酰，ODR-10的天然配体。此外，生物工程OSNs显示不同的时间放电模式，在响应不同浓度的双乙酰。所有的结果表明，生物工程的OSNs是有用的，并有希望作为一种新型的传感元件的生物传感器，用于特定的气味分子的检测。生物工程技术为嗅觉敏感元件的制备提供了新的途径，并将促进嗅觉生物传感器的发展。(C)2012爱思唯尔有限公司版权所有。
It is highly desirable to obtain functional cells with specific olfactory receptors (ORs) for the development of cell-based biosensors towards odorant detection. In this study, we explored the feasibility of bioengineered primary olfactory sensory neurons (OSNs) as sensing elements of biomimetic olfactory-based biosensors, in which light addressable potentiometric sensor (LAPS) was used to monitor bioengineered OSNs membrane potential responses to odorant molecules. An olfactory receptor of C. elegances, ODR-10, as a model receptor, was expressed on the plasma membrane of OSNs by transient transfection. The response profile of bioengineered OSNs to odorant molecules was investigated by analyzing extracellular potential firings features in frequency and time domains. The results indicated that bioengineered OSNs can specifically respond to diacetyl, the natural ligand of ODR-10. In addition, bioengineered OSNs showed different temporal firing patterns in responding to different concentrations of diacetyl. All the results demonstrate that bioengineered OSNs are useful and promising to serve as novel sensing elements of biosensors for specific odorant molecule detection. It is suggested that bioengineering techniques could provide novel approaches for preparing sensitive elements as well as promoting the development of practical applicable olfactory-based biosensors. (C) 2012 Elsevier B.V. All rights reserved.