Small ubiquitin-like modifier conjugating enzyme with active site mutation acts as dominant negative inhibitor of SUMO conjugation in arabidopsis(F).
Small ubiquitin-like modifier conjugating enzyme with active site mutation acts as dominant negative inhibitor of SUMO conjugation in arabidopsis(F).
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DOI：
10.1111/jipb.12016
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发表时间：
2013
影响因子：
11.4
通讯作者：
Konstantin Tomanov;C. Hardtke;R. Budhiraja;Rebecca Hermkes;G. Coupland;A. Bachmair
中科院分区：
文献类型：
--
作者：
Konstantin Tomanov;C. Hardtke;R. Budhiraja;Rebecca Hermkes;G. Coupland;A. Bachmair
小泛素样修饰物（SUMO）的缀合影响植物的广泛过程，包括生长、花的起始、病原体防御和对非生物胁迫的响应。在这里，我们调查在体内和体外的SUMO共轭酶与半胱氨酸丝氨酸的变化，在活性位点，并表明，它具有显着的负面影响。在植物中的表达显著干扰正常发育，导致生长迟缓，早期开花和基因表达的变化。我们认为，突变蛋白可以作为一个探针，调查sumoylation，也在植物的遗传基础设施差，排除通过功能丧失突变体的分析。
Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.