A new emerging serotype of Vibrio parahaemolyticus in China is rapidly becoming the main epidemic strain
A new emerging serotype of Vibrio parahaemolyticus in China is rapidly becoming the main epidemic strain
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中国新出现的副溶血性弧菌血清型正迅速成为主要流行毒株
DOI：
10.1016/j.cmi.2019.09.024
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发表时间：
2020-05-01
影响因子：
14.2
通讯作者：
Chen, Y.
中科院分区：
文献类型：
--
作者：
Chen, X.;Li, Y.;Chen, Y.
目的：在监测过程中发现一种新型副溶血弧菌，命名为“O4:KUT-recAin”，并研究其表型、致病性和流行病学特征。方法：从我国沿海医院急性腹泻患者中分离副溶血弧菌。采用血清凝集试验、特异性PCR检测、不同条件下的生长曲线和兔腹泻模型对O4:KUT-recAin进行表征。结果：O4:KUT-recAin菌株具有新型K抗原，并在管家基因recA中插入了编码20个蛋白的25 043 bp大片段。回顾性分析发现，2014年563株副溶血性弧菌中仅检出1株O4:KUT-recAin；此后比例迅速上升，2016年达到17.8%（105/590），2017年达到31.1%（224/721），使O4:KUT-recA成为继O3:K6之后的第二个优势血清型。 O4:KUT-recAin 菌株 (100%, 14/14) 表现出增强的耐酸性，并且可以在 pH 4.9 的培养基中繁殖，而 92.9% (13/14) 的 O3:K6 菌株在此 pH 值下无法生长。 O4:KUT-recAin可引起幼兔腹泻和小肠组织病变，但其腹泻率(93.1%，27/29)和死亡率(78.6%，22/28)略低于O3:K6(100%16/16，100%16/16)。基于腹泻患者，除培养基年龄、血红蛋白和粪便样本中红细胞数量外，两组的大多数临床症状和实验室结果均无显着差异。 结论：O4:KUT-recAin具有增强的耐酸性，能够引起兔子和人类的感染性腹泻，并在短时间内在中国广泛流行。 (C) 2019 年欧洲临床微生物学和传染病学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: During surveillance, we found a new type of Vibrio parahaemolyticus named 'O4:KUT-recAin' and studied the phenotypic, pathogenic and epidemiological characteristics of O4:KUT-recAin.Methods: V. parahaemolyticus were isolated from acute diarrhoeal patients in coastal hospitals of China. Serum agglutination test, specific PCR assay, growth curves under different conditions and rabbit diarrhoeal models were using to characterize O4:KUT-recAin.Results: The O4:KUT-recAin strain has a new type of K antigen and a 25 043-bp-large fragment encoding 20 proteins inserted in the housekeeping gene recA. Retrospective analysis found that only one O4:KUT-recAin strain was detected in 563 V. parahaemolyticus strains in 2014; then the proportion increased rapidly and reached 17.8% (105/590) in 2016 and 31.1% (224/721) in 2017, making O4:KUT-recAin the second dominant serotype following O3:K6. O4:KUT-recAin strains (100%, 14/14) exhibited increased acid resistance and could reproduce in medium at pH 4.9, while 92.9% (13/14) of the O3:K6 strains could not grow at this pH value. O4:KUT-recAin could cause diarrhoea and small intestinal tissue lesions in infant rabbits, but its diarrhoeal (93.1%, 27/29) and mortality (78.6%, 22/28) rates were slightly lower than those of O3:K6 (100% 16/16, 100% 16/16). Based on diarrhoea patients, there were no significant differences in the two groups for most clinical symptoms and laboratory results, except media age, haemoglobin and the number of red blood cells in stool samples.Conclusions: O4:KUT-recAin had enhanced acid resistance, was capable of causing infectious diarrhoea in both rabbits and humans, and has become widespread during a short period of time in China. (C) 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.