Kinetics of CO Recombination to the Heme in Geobacillus Stearothermophilus Nitric Oxide Synthase.
Kinetics of CO Recombination to the Heme in Geobacillus Stearothermophilus Nitric Oxide Synthase.
复制标题
嗜热脂肪芽孢杆菌一氧化氮合酶中 CO 重组为血红素的动力学。
DOI：
10.1016/j.poly.2012.08.079
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发表时间：
2013
期刊：
影响因子：
2.6
通讯作者：
Gray,HarryB
中科院分区：
文献类型：
--
作者：
Whited,CharlotteA;Warren,JeffreyJ;Lavoie,KatherineD;Winkler,JayR;Gray,HarryB
我们报道了嗜热地脂杆菌一氧化氮合酶（gsNOS）的His134Ser、Ile223Val和His134Ser/Ile223Ser突变体中CO与血红素的再结合动力学。两个观察到的动力学相的振幅对CO浓度不敏感，取决于酶的浓度。我们认为，在所采用的条件下（6.1-27μM gsNOS与20%或100% CO气氛），两种形式的gsNOS处于平衡状态。一氧化碳再结合到血红素的动力学不依赖于位置134和223的NO-gate残基的身份。
We report the kinetics of CO rebinding to the heme in His134Ser, Ile223Val and His134Ser/Ile223Ser mutants of Geobacillus stearothermophilus nitric oxide synthase (gsNOS). The amplitudes of the two observed kinetics phases, which are insensitive to CO concentration, depend on enzyme concentration. We suggest that two forms of gsNOS are in equilibrium under the conditions employed (6.1–27μM gsNOS with 20 or 100% CO atmosphere). The kinetics of CO rebinding to the heme do not depend on the identity of the NO-gate residues at positions 134 and 223.