High frequency of circulating follicular helper T cells Is correlated with B cell subtypes in patients with ankylosing spondylitis
High frequency of circulating follicular helper T cells Is correlated with B cell subtypes in patients with ankylosing spondylitis
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DOI：
10.3892/etm.2018.5991
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发表时间：
2018-05-01
影响因子：
2.7
通讯作者：
Shang, Xianwen
中科院分区：
文献类型：
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作者：
Long, Siqi;Ma, Li;Shang, Xianwen
已知T滤泡辅助细胞（Tfh）支持效应IB细胞并增强自身免疫；然而，强直性脊柱炎（AS）中Tfh细胞和B细胞之间的关系尚不清楚。本研究的目的是测量AS患者外周血cd4 (+)C-X-C趋化因子受体5型（CXCR5）（+） Tfh细胞和IB细胞亚型的循环频率，并评估这些因素的相关性。通过流式细胞术检测外周血循环CD4(+)CXCR5(+) Tfh细胞和B细胞亚型的百分比，使用Bath AS疾病活动指数（BASDAI）检测个体患者的疾病活动性。通过Spearman’s或Pearson’s相关性分析这些测量之间的潜在关联。与健康对照（HC）相比，AS患者CD4(+)CXCR5(+)cTfh、CD4(+)CXCR5（+）程序性死亡1（+）、CD4(+)CXCR5（+）诱导T细胞共刺激器（ICOS）（+）、CD3(+)CD8(-)CXCR5（+）白细胞介素(IL)-21(+) T细胞、CD19(+)CD27（高）浆母细胞和CD19(+)CD38（+）抗体分泌B细胞的百分比显著增加，而CD19(+)CD27（-）初始B细胞和CD19(+)CD27（+）记忆B细胞的百分比无显著差异。将AS患者分为高活性组和低活性组，高活性AS组CD4(+)CXCR5（+）、CD3(+)CD8(-)CXCR5(+)IL-21(+) T细胞、CD19(+)CD27（-）幼稚B细胞和CD19(+)CD38（+）抗体分泌B细胞的百分比明显高于低活性AS组，CD19(+)CD27（+）记忆B细胞的百分比明显低于低活性AS组。此外，CD4(+)CXCR5（+）循环(c)Tfh、CD3(+)CD8(-)CXCR5(+)TL-21(+) T和CD19(+)CD38（+）抗体分泌B细胞的百分比与BASDAI值呈正相关。此外，AS患者CD4+ CXCR5+ cTfh细胞的百分比与CD19+ CD38+抗体分泌B细胞的百分比呈正相关，CD3(+)CD8(-)CXCR5(+)IL-21(+)T细胞的百分比与CD19 (+)CD27（-）幼稚B细胞的百分比呈正相关。这些发现提示CD4+ CXCR5(+) cTfh、CD3(+)CD8-CXCR5(+)IL-21(+) T和CD19(+)CD38（+）抗体分泌B细胞由于其不同的功能可能参与了AS的发病过程。因此，cTfh和B细胞亚型的水平是评估As患者疾病活动性的有用生物标志物。
T follicular helper (Tfh) cells are known to support effector IB cells and enhance autoimmunity; however, the association between the Tfh cells and B cells in ankylosing spondylitis (AS) is unclear. The aim of the present study was to measure the frequency of circulating cluster of differentiation (CD)4(+)C-X-C chemokine receptor type 5 (CXCR5)(+) Tfh cells and IB cell subtypes in peripheral blood from patients with AS, and evaluate the correlation of these factors. Percentages of peripheral blood circulating CD4(+)CXCR5(+) Tfh cells and B cell subtypes were measured via flow cytometry and the disease activity of individual patients was measured using the Bath AS Disease Activity Index (BASDAI). The potential association among these measures was analyzed via Spearman's or Pearson's correlations. In comparison with those in healthy controls (HC), significantly increased percentages of CD4(+)CXCR5(+)cTfh, CD4(+)CXCR5(+)programmed death 1(+), CD4(+)CXCR5(+) inducible T cell costimulator (ICOS)(+), CD3(+)CD8(-)CXCR5(+)interleukin(IL)-21(+) T cells, CD19(+) CD27(high) plasmablast and CD19(+)CD38(+) antibody-secreting B cells were detected in patients with AS, whereas there was no significant difference in CD 19(+)CD27(-) naive B cells and CD19(+)CD27(+) memory B cells. When Patients with AS were divided into high and low activity groups, significantly higher percentages of CD4(+)CXCR5(+), CD3(+)CD8(-)CXCR5(+)IL-21(+) T cells, CD19(+)CD27(-) naive B cells and CD19(+)CD38(+) antibody-secreting B cells, and lower CD19(+)CD27(+) memory B cells were detected in high activity AS group compared with the low activity AS group. In addition, percentages of CD4(+)CXCR5(+) circulating (c)Tfh, CD3(+)CD8(-)CXCR5(+)TL-21(+) T and CD19(+)CD38(+) antibody-secreting B cells were positively correlated with BASDAI values. Furthermore, the percentage of CD4+ CXCR5+ cTfh cells was positively correlated with CD19+ CD38+ antibody-secreting B cells and the percentage of CD3(+)CD8(-)CXCR5(+)IL-21(+)T cells was positively correlated with CD 19(+)CD27(-) naive B cells in patients with AS. These findings suggest that CD4+ CXCR5(+) cTfh, CD3(+)CD8-CXCR5(+)IL-21(+) T and CD19(+)CD38(+) antibody-secreting B cells may participate in the pathogenesis of AS because of their distinct functions. As such, levels of cTfh and B cell subtypes ma) be a useful biomarker for the evaluation of disease activity in patients with AS.