Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks
Melatonin modulates monochromatic light-induced melatonin receptor expression in the hypothalamus of chicks
复制标题
褪黑素调节雏鸡下丘脑单色光诱导的褪黑素受体表达
DOI：
10.1016/j.acthis.2017.09.005
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发表时间：
2017-01-01
影响因子：
2.5
通讯作者：
Chen, Yaoxing
中科院分区：
文献类型：
--
作者：
Zhang, Liwei;Chen, Funing;Chen, Yaoxing
为了研究单色光对雏鸡生理功能的影响机制，将192只刚孵化的雏鸡随机分为完整组、假手术组和松果体切除组，分别在发光二极管（LED）系统下照射白光（WL）、红光（RL）、绿光（GL）和蓝光（BL）两周。在P14时，立即收集下丘脑进行褪黑激素受体亚型（Melia和Mellb）的免疫组化染色，并使用RT-PCR和western blot检测Mella和Mellb的表达。下丘脑免疫组化染色显示Melib-ir细胞分布于视前区（POA）、视前脑室周围核（POP）和视交叉上核（SCN），在POA和SCN均可见Melib-ir细胞。RT-PCR和western blot分析显示，与对照组相比，GL处理的下丘脑Mella和Mellb mRNA和蛋白水平分别提高了10.7-29.3% （P = 0.029-0.002）、9.18-35.9%和8.97-27.3% （P = 0.027-0.001）、BL （P = 0.038-0.007）。然而，松果体切除术后，这些参数下降，WL、RL、GL和BL组之间无显著差异。这些结果表明，褪黑激素在GL光照下增强鸡下丘脑Mella和Mellb的表达中起关键作用。
To study the mechanism of the effect of monochromatic light on physiological function in chicken, a total of 192 newly hatched chicks were randomly divided into intact, sham-operated and pinealectomy groups then exposed to white light (WL), red light (RL), green light (GL) and blue light (BL) using a light-emitting diode (LED) system for two weeks. At P14, the hypothalami were immediately collected for immunohistochemical staining of melatonin receptor subtypes (Melia and Mellb) and detection of Mella and Mellb expressions using RT-PCR and western blot. Immunohistochemical staining of the hypothalamus showed that the Mella-ir cells were distributed in the preoptic area (POA), nucleus preopticus periventricularis (POP) and suprachiasmatic nuclei (SCN), and the Melib-ir cells were presented in the POA and SCN. Analysis of RT-PCR and western blot showed that the mRNA and protein levels of Mella and Mellb in the hypothalamus of chick exposed to GL were increased by 10.7-29.3%, 9.18-35.9% and 8.97-27.3% compared to those in the chicks exposed to WL (P = 0.029-0.002), RL (P = 0.027-0.001) and BL (P = 0.038-0.007) in the intact group, respectively. After pinealectomy, however, these parameters decreased and there were no significant differences among the WL, RL, GL and BL groups. These findings suggested that melatonin plays a critical role in GL illumination-enhanced Mella and Mellb expressions in the hypothalamus of chicks.