Natural Variation, Functional Pleiotropy and Transcriptional Contexts of Odorant Binding Protein Genes in Drosophila melanogaster
Natural Variation, Functional Pleiotropy and Transcriptional Contexts of Odorant Binding Protein Genes in Drosophila melanogaster
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DOI：
10.1534/genetics.110.123166
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发表时间：
2010-12-01
期刊：
影响因子：
3.3
通讯作者：
Anholt, Robert R. H.
中科院分区：
文献类型：
--
作者：
Arya, Gunjan H.;Weber, Allison L.;Anholt, Robert R. H.
功能多样性如何影响转录组的组织是系统遗传学中的一个中心问题。为了探讨这个问题，我们测序了所有六个气味结合蛋白（Obp）基因位于X染色体上，其中四个出现作为一个集群，在219近交野生衍生系的果蝇和测试之间的关联遗传和表型变异在有机体和转录水平。我们观察到Obp8a、Obp19a、Obp19b和Obp19c的多态性与嗅觉反应的变化相关，Obp19d的多态性与寿命的变化相关。我们推断的转录背景下，或“生态位”，每个基因的表达多态性，在这些Obp基因的遗传变异与遗传相关的每个Obp基因的转录本的表达变化。所有六个Obp基因占据了一个独特的转录生态位。基因本体富集分析揭示了不同的Obp转录小生境与嗅觉行为，突触传递，检测信号调节组织发育和细胞凋亡，交配后行为和产卵，和营养传感的协会。我们的研究结果表明，多样化的Obp家族有组织的独特的转录生态位，反映了他们收购的额外功能。
How functional diversification affects the organization of the transcriptome is a central question in systems genetics. To explore this issue, we sequenced all six Odorant binding protein (Obp) genes located on the X chromosome, four of which occur as a cluster, in 219 inbred wild-derived lines of Drosophila melanogaster and tested for associations between genetic and phenotypic variation at the organismal and transcriptional level. We observed polymorphisms in Obp8a, Obp19a, Obp19b, and Obp19c associated with variation in olfactory responses and polymorphisms in Obp19d associated with variation in life span. We inferred the transcriptional context, or "niche," of each gene by identifying expression polymorphisms where genetic variation in these Obp genes was associated with variation in expression of transcripts genetically correlated to each Obp gene. All six Obp genes occupied a distinct transcriptional niche. Gene ontology enrichment analysis revealed associations of different Obp transcriptional niches with olfactory behavior, synaptic transmission, detection of signals regulating tissue development and apoptosis, postmating behavior and oviposition, and nutrient sensing. Our results show that diversification of the Obp family has organized distinct transcriptional niches that reflect their acquisition of additional functions.