Acute Oral Toxicity of Tetrodotoxin in Mice: Determination of Lethal Dose 50 (LD50) and No Observed Adverse Effect Level (NOAEL).
Acute Oral Toxicity of Tetrodotoxin in Mice: Determination of Lethal Dose 50 (LD50) and No Observed Adverse Effect Level (NOAEL).
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DOI：
10.3390/toxins9030075
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发表时间：
2017-02-24
期刊：
影响因子：
4.2
通讯作者：
Botana LM
中科院分区：
文献类型：
--
作者：
Abal P;Louzao MC;Antelo A;Alvarez M;Cagide E;Vilariño N;Vieytes MR;Botana LM
河豚毒素（TTX）开始出现在欧洲沃茨的软体动物中，对海产品消费者构成危害。这种毒素阻断钠通道，导致神经肌肉麻痹，甚至死亡。作为导致TTX安全海鲜水平的风险评估过程的一部分，需要口服毒性数据。在本研究中，设计了一个4水平的升降程序，以便通过使用准确的良好表征的TTX标准品首次确定小鼠的口服致死剂量50（LD 50）和无明显不良作用水平（NOAEL）。
Tetrodotoxin (TTX) is starting to appear in molluscs from the European waters and is a hazard to seafood consumers. This toxin blocks sodium channels resulting in neuromuscular paralysis and even death. As a part of the risk assessment process leading to a safe seafood level for TTX, oral toxicity data are required. In this study, a 4-level Up and Down Procedure was designed in order to determine for the first time the oral lethal dose 50 (LD50) and the No Observed Adverse Effect Level (NOAEL) in mice by using an accurate well-characterized TTX standard.