Visualization of in vitro protein-rejecting properties of PEGylated stealth® polycyanoacrylate nanoparticles
Visualization of in vitro protein-rejecting properties of PEGylated stealth® polycyanoacrylate nanoparticles
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DOI：
10.1016/s0142-9612(99)00021-6
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发表时间：
1999-07-01
期刊：
影响因子：
14
通讯作者：
Couvreur, P
中科院分区：
文献类型：
--
作者：
Peracchia, MT;Harnisch, S;Couvreur, P
在将纳米粒子与作为一种血液蛋白模型的纤维蛋白原预孵育后，对其进行冷冻断裂，首次观察到聚乙二醇（PEG）包被的聚氰基丙烯酸烷基酯（PACA）纳米粒子在体外的蛋白质排斥特性。在将纳米粒子与人血浆孵育后，通过二维聚丙烯酰胺凝胶电泳（PAGE）也证明了蛋白质与纳米粒子的结合减少。体内实验表明，聚乙二醇化纳米粒子在静脉注射给小鼠后具有“隐形”的长循环特性。因此，纳米粒子 - 蛋白质孵育后获得的图像可预测体内观察到的行为。总之，冷冻断裂分析是一种新颖且独特的定性方法，可用于研究蛋白质与微粒系统之间的相互作用。（C）1999年爱思唯尔科学有限公司。保留所有权利。
The in vitro protein-rejecting properties of PEG-coated polyalkylcyanoacrylate (PACA) nanoparticles were for the first time visualized after freeze-fracture of the nanoparticles pre-incubated with fibrinogen as a model blood protein. The reduced protein association to the nanoparticles was evidenced also by two-dimensional PAGE after incubation of the nanoparticles with human plasma. In vivo experiments showed the 'stealth' long-circulating properties of the PEGylated nanoparticles after intravenous administration to mice. Thus, the images obtained after nanoparticle-protein incubation were predictive of the behavior observed in vivo. In conclusion, freeze-fracture analysis represents a novel and original qualitative approach. to investigate the interactions between proteins and particulate systems. (C) 1999 Elsevier Science Ltd. All rights reserved.