Modeling human development in 3D culture
Modeling human development in 3D culture
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DOI：
10.1016/j.ceb.2014.06.013
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发表时间：
2014-12-01
影响因子：
7.5
通讯作者：
Tanaka, Elly M.
中科院分区：
文献类型：
--
作者：
Ader, Marius;Tanaka, Elly M.
近年来，人类胚胎干细胞研究进入了一个新的维度--第三维。利用关于沿着沿着不同谱系引导多能细胞的知识的增加，结合ECM支持的三维培养条件，已经可以产生中枢神经系统、肠、肝和肾的高度组织化的组织。每个系统已被用于研究器官发生和功能的不同方面，包括视杯形态发生的物理力，祖细胞控制中疾病相关基因的功能，以及移植后所产生的组织与宿主组织的相互作用。多能干细胞衍生的类器官代表了用于研究细胞如何自组织以产生具有给定形状、模式和形式的组织的强大系统。
Recently human embryonic stem cell research has taken on a new dimension-the third dimension. Capitalizing on increasing knowledge on directing pluripotent cells along different lineages, combined with ECM supported three-dimensional culture conditions, it has become possible to generate highly organized tissues of the central nervous system, gut, liver and kidney. Each system has been used to study different aspects of organogenesis and function including physical forces underlying optic cup morphogenesis, the function of disease related genes in progenitor cell control, as well as interaction of the generated tissues with host tissue upon transplantation. Pluripotent stem cell derived organoids represent powerful systems for the study of how cells self-organize to generate tissues with a given shape, pattern and form.