Developmental potential of cryopreserved gonadal germ cells from 7-day-old chick embryos recovered using the PBS(-) method
Developmental potential of cryopreserved gonadal germ cells from 7-day-old chick embryos recovered using the PBS(-) method
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DOI：
10.1080/00071668.2021.1960952
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发表时间：
2021-08-20
影响因子：
2
通讯作者：
Tajima, A.
中科院分区：
文献类型：
--
作者：
Nakajima, Y.;Asano, A.;Tajima, A.
1. 使用 PBS(-) 方法进行了一系列实验，以检查从胚胎第 7 天的雄性和雌性胚胎中回收的冷冻保存的性腺生殖细胞 (GGC) (7 d-GGC) 的发育潜力。通过将从雌性/雄性罗德岛红 (RIR) 胚胎中回收的 200 个冷冻/未冷冻的 7 d-GGC 转移到 2 天大的雌性和雄性白来航 (WL) 胚胎的背主动脉中，产生种系嵌合体。 2.将生殖细胞受体胚胎孵化并培养至性成熟，并通过与异性RIR交配进行后代测试。除了通过转移雌性 GGC 产生的雄性 GGC 受体外，在所有八种可能的后代测试组合中孵化出棕色羽毛的后代雏鸡。此外，当通过转移未冷冻的7d-GGC产生的雄性种系嵌合体的冷冻解冻精子在正常雌性RIR和雌性WL种系嵌合体中受精时，孵化出棕色羽毛的后代雏鸡。 3.结果表明，使用PBS(-)方法回收的7日龄鸡胚冷冻保存的雌性/雄性GGC在适当的GGC供体/受体组合下完全能够在受体胚胎的性腺中发育成正常的精子和卵子。
1. A series of experiments were conducted to examine the developmental potential of cryopreserved gonadal germ cells (GGCs) recovered from both males and females on embryo day 7 (7 d-GGCs) using the PBS(-) method. Germline chimeras were produced by transferring 200 frozen/unfrozen 7 d-GGCs recovered from female/male Rhode Island Red (RIR) embryos into the dorsal aorta of 2-day-old female and male white leghorn (WL) embryos. 2. Germ-cell recipient embryos were hatched and raised to sexual maturity and progeny testing was conducted by mating with RIR of the opposite sex. Brown-feathered progeny chicks were hatched in all eight possible progeny testing combinations, except for male GGC recipients produced by transferring female GGCs. Furthermore, brown-feathered progeny chicks were hatched when frozen-thawed sperm from male germline chimeras, produced by transferring unfrozen 7d-GGCs, were inseminated in normal female RIR and female WL germline chimeras. 3. The results indicated that cryopreserved female/male GGCs from 7-day-old chick embryos, recovered using the PBS(-) method, were fully capable of developing into normal spermatozoa and ova in the gonad of recipient embryos under appropriate GGC donor/recipient combinations.