Oxidative stress, DNA damage, and cellular response in hydrogen peroxide-induced cell injury of mud crab (Scylla paramamosain).
Oxidative stress, DNA damage, and cellular response in hydrogen peroxide-induced cell injury of mud crab (Scylla paramamosain).
复制标题
DOI：
10.1016/j.fsi.2021.04.015
复制
发表时间：
2021-04
影响因子：
4.7
通讯作者：
Changhong Cheng;Hongling Ma;Guangfeng Liu;Yiqin Deng;Juan Feng;Yu-Kun Jie;Zhixun Guo
中科院分区：
文献类型：
--
作者：
Changhong Cheng;Hongling Ma;Guangfeng Liu;Yiqin Deng;Juan Feng;Yu-Kun Jie;Zhixun Guo
氧化应激被认为是环境应激源对水生生物的毒性机制。本研究旨在探讨氧化应激对青蟹生理反应、DNA损伤和转录谱的影响。将0.1%和1%的过氧化氢(H_2O_2)分别注入泥蟹体内72 h，结果表明，注入H_2O_2后，泥蟹体内的超氧化物歧化酶和过氧化氢酶活性显著降低。注射H_2O_2后，丙二醛含量、H_2O_2含量、天冬氨酸氨基转移酶、丙氨酸氨基转移酶和乳酸脱氢酶活性均显著升高。此外，在注射双氧水后，DNA发生了损伤。转录组分析表明，0.1%和1%双氧水注射后，分别鉴定出531个和372个差异表达基因(Deg)。这些DEGS主要参与氧化应激反应和免疫功能。所有这些结果表明，氧化应激可以损害抗氧化防御系统和免疫系统。转录组分析提供了与泥蟹氧化应激反应相关的基因功能的有价值的信息。
Oxidative stress is considered as the toxicity mechanism of environmental stressors on aquatic organisms. This study aims to explore the effects of oxidative stress on physiological responses, DNA damage and transcriptional profiles of the mud crabsScylla paramamosain. In the present study, mud crabs were injected with 0.1% and 1% hydrogen peroxide (H2O2) for 72 h. The results showed that superoxide dismutase and catalase activities significantly decreased after H2O2injection. Malondialdehyde content, H2O2content, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activity significantly increased after H2O2injection. Moreover, DNA damage occurred after H2O2injection. Transcriptome analysis showed that 531 and 372 differentially expressed genes (DEGs) were identified after 0.1% and 1% H2O2injection, respectively. These DEGs were mainly involved in the oxidative stress response and immune functions. All these results indicated that oxidative stress could impair both antioxidant defense systems and immune systems. Transcriptome analysis provided valuable information on gene functions associated with the response to oxidative stress in the mud crab.