GUINEA-PIG PREPROINSULIN GENE - AN EVOLUTIONARY COMPROMISE
GUINEA-PIG PREPROINSULIN GENE - AN EVOLUTIONARY COMPROMISE
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DOI：
10.1073/pnas.81.16.5046
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
EFSTRATIADIS, A
中科院分区：
文献类型：
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作者：
CHAN, SJ;EPISKOPOU, V;EFSTRATIADIS, A
一个克隆携带豚鼠前胰岛素原基因的特点，这与其他哺乳动物前胰岛素原基因，是高度不同的，在其区域编码的成熟胰岛素的B和A链。印迹杂交分析表明，该基因在豚鼠基因组中仅以1个拷贝存在，其他正常或突变的前胰岛素原基因在该动物中不存在。该基因内含子的位置及其3“侧翼区与其它已测序哺乳动物基因相应区域的同源性表明，该基因来源于普通哺乳动物。根据序列趋异分析，编码B和A链的区域的快速进化可以解释为由于中性和适应性突变的固定。
A clone carrying the guinea pig preproinsulin gene was characterized, which, in contrast to other mammalian preproinsulin genes, is highly divergent in its regions encoding the B and A chains of mature insulin. Blot hybridization analysis indicates that this gene is present in only 1 copy in the guinea pig genome and that other normal or mutated preproinsulin genes do not exist in this animal. The position of introns in this gene and the homology of its 3'' flanking region to the corresponding regions of other sequenced mammalian genes show that it was derived from the common mammalian stock. The rapid evolution of the region encoding the B and A chains can be interpreted, according to a sequence-divergence analysis, as due to the fixation of both neutral and adaptive mutations.