Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation
Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation
复制标题
哺乳动物细胞中潜在致命辐射损伤的修复与恶性转化的增强有关
作者：
Margaret Terzaghi;J. Little
已经进行了多次尝试来确定在体外哺乳动物细胞中固定转化状态所需的条件。在暴露于甲基胆蒽1、X射线照射2、3和SV40病毒4的细胞培养物中研究了处理后固定和增殖之间的关系。细胞在生长的密度抑制平台期暴露于致癌物，随后以低细胞密度传代培养，以便它们能够恢复细胞分裂。通过延迟继代培养 24 小时或更长时间，转化频率总是会降低，这表明细胞在接触致癌物后必须立即分裂，以固定转化状态。治疗后 24 小时内没有报告继代培养的数据。
THERE have been several attempts to define conditions necessary for the fixation of the transformed state in mammalian cells in vitro. The relationship between fixation and proliferation after treatment has been studied in cell cultures exposed to methylcholanthrene1, X irradiation2,3 and SV40 virus4. Cells were exposed to the carcinogen while in the density-inhibited plateau phase of growth, and subsequently subcultured at low cell density so that they could resume cell division. Transformation frequency was always reduced by delaying subculture for 24 h or longer, suggesting that cells must divide soon after exposure to a carcinogen in order to fix the transformed state. No data were reported for subculture less than 24 h after treatment.