DIVERSITY OF BORRELIA-BURGDORFERI SENSU LATO EVIDENCED BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF RRF (5S) RRL (23S) INTERGENIC SPACER AMPLICONS
DIVERSITY OF BORRELIA-BURGDORFERI SENSU LATO EVIDENCED BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM OF RRF (5S) RRL (23S) INTERGENIC SPACER AMPLICONS
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DOI：
10.1099/00207713-44-4-743
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发表时间：
1994-10-01
期刊：
影响因子：
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通讯作者：
BARANTON, G
中科院分区：
文献类型：
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作者：
POSTIC, D;ASSOUS, MV;BARANTON, G
核糖体基因的组织在伯氏疏螺旋体中是独特的，因为rrl（23 S）和rrf（5S）基因串联复制。我们利用这种独特性来评估在第一个rrf基因的3'末端和第二个rrl基因的5'末端用引物获得的PCR产物的限制性多态性。从100个B中的99个产生226至266 bp长的扩增子。伯氏菌晚期菌株。扩增子的核酸酶MseI限制性多态性为鉴定B提供了有用的工具。严格意义上的伯氏疏螺旋体、伽氏疏螺旋体、阿氏疏螺旋体（原VS 461组）和日本疏螺旋体（原F63 B组）。此外，它使我们能够识别四个新的基因组群，这是由DNA-DNA杂交数据证实。其中两组基因组包含欧洲菌株，另外两组包含美国菌株。美洲的基因组群涉及的载体具有与B完全不同的地方流行周期。严格意义上的伯氏疏螺旋体（burgdorferi sensu stricto），其先前是已知在美国发生的唯一莱姆病疏螺旋体物种。该方法可用于菌株的快速筛选以及流行病学和医学目的。
The organization of the ribosomal genes is unique in Borrelia burgdorferi in that the rrl (23S) and rrf (5S) genes are tandemly duplicated. We took advantage of this uniqueness to assess the restriction polymorphism of PCR products obtained with primers at the 3' end of the first rrf gene and at the 5' end of the second rrl gene. An amplicon that was 226 to 266 bp long was generated from 99 of 100 B. burgdorferi sensu late strains. The nuclease MseI restriction polymorphism of the amplicons provided a useful tool for identifying B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii (formerly group VS461), and Borrelia japonica (formerly group F63B). Furthermore, it allowed us to recognize four new genomic groups, which were confirmed by DNA-DNA hybridization data. Two of these genomic groups comprised European strains, and the other two groups contained American strains. The American genomic groups involved vectors with enzootic cycles quite different from those of B. burgdorferi sensu stricto, which previously was the only Lyme disease Borrelia species known to occur in the United States. Our method could be used for rapid screening of strain collections and for epidemiological and medical purposes.