Hemocompatibility and anaphylatoxin formation of protein-immobilizing polyacrylonitrile hemodialysis membrane
Hemocompatibility and anaphylatoxin formation of protein-immobilizing polyacrylonitrile hemodialysis membrane
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DOI：
10.1016/j.biomaterials.2004.04.039
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发表时间：
2005-04-01
期刊：
影响因子：
14
通讯作者：
Yang, MC
中科院分区：
文献类型：
--
作者：
Liu, TY;Lin, WC;Yang, MC
将血浆蛋白共价固定在聚丙烯腈（PAN）膜上，以评价血液相容性和过敏毒素形成。这是用来作为一个模型，研究蛋白质吸附的血液透析膜的血液接触反应的影响。使用的蛋白质是血小板粘附促进胶原（COL）或血小板粘附抑制人血清白蛋白（HSA）。通过考马斯染料分析、原子力显微镜、X射线光电子能谱和水接触角测量等手段对PAN膜的微观结构和表征进行了研究。PAN-HSA膜不仅改善了血液相容性，包括减少血小板粘附，延长凝血时间，提高凝血酶活性水平，而且诱导了较低的补体激活。另一方面，PAN COL膜具有血液不相容性，但引起的血清C3、C4抗原的增加较小。本研究的总体结果表明，将HSA固定在PAN膜表面将有利于改善血液相容性，并减少血液透析过程中过敏毒素的形成。(C)2004爱思唯尔有限公司保留所有权利。
Plasma proteins were covalently immobilized onto polyacrylonitrile (PAN) membrane to evaluate the hemocompatibility and anaphylatoxin formation. This is used as a model to study the effect of protein-adsorption on the blood-contacting response of hemodializing membranes. The proteins used were either platelet-adhesion-promoting collagen (COL) or platelet-adhesion-inhibiting human serum albumin (HSA). The microstructure and characterization of the protein-immobilizing PAN membranes were evaluated by Coomassie dye assay, atomic force microscopy, X-ray photoelectron spectroscopy and water contact angle measurement. PAN-HSA membrane improved not only hemocompatibility including less platelet adhesion, longer blood coagulation times, and higher thrombin inactivity level, but also induced lower complement activation. On the other hand, PAN COL membrane exhibited blood incompatibility, although induced less increase of C3, C4 antigens of serum. Overall results of this study demonstrated that the immobilization of HSA onto the surface of PAN membrane would be beneficial to improve the hemocompatibility and to reduce the anaphylatoxin formation during hemodialysis. (C) 2004 Elsevier Ltd. All rights reserved.