FGF-2-Responsive and Spinal Cord-Resident Cells Improve Locomotor Function after Spinal Cord Injury
FGF-2-Responsive and Spinal Cord-Resident Cells Improve Locomotor Function after Spinal Cord Injury
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DOI：
10.1089/neu.2009.1108
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发表时间：
2014-09-15
影响因子：
4.2
通讯作者：
Furukawa, Shoei
中科院分区：
文献类型：
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作者：
Kasai, Masaki;Jikoh, Takahiro;Furukawa, Shoei
成人中枢神经系统的轴突再生能力有限。本研究采用大鼠脊髓全横断后，将成纤维细胞生长因子-2(FGF2)一次性注入损伤部位周围的脊髓组织。该疗法可明显改善动物的运动功能。组织学分析显示，由成纤维细胞生长因子-2诱导的纤维连接蛋白阳性细胞(FIFs)组成的组织已渗入损伤部位并填充大的囊腔，大量具有生长相关蛋白-43免疫反应的轴突穿透其中。FIF也可以从完整的脊髓组织中培养出来，表明它们存在于未损伤的脊髓中。细胞形态呈纺锤形，N-钙粘蛋白和神经营养因子的mRNAs表达增强，提示FIFF具有促进轴突再生的作用。因此，这些结果支持继续使用自体移植作为一种新的和有前途的细胞疗法来治疗脊髓损伤。
The adult central nervous system has only a limited capacity for axonal regeneration. In this study, fibroblast growth factor-2 (FGF-2) was injected once into the spinal cord tissue around the injury site immediately after complete spinal cord transection in rats. This treatment markedly improved the locomotor function of the animals. Histological analysis demonstrated that tissue composed of FGF-2-induced fibronectin-positive cells (FIFs) had infiltrated the injury site and filled large cystic cavities, into which numerous axons with growth-associated protein-43 immunoreactivity penetrated. The FIFs could also be cultured from the intact spinal cord tissue, demonstrating that they were resident in the non-injured spinal cord. They had a spindle-shaped morphology and enhanced expression of mRNAs of N-cadherin and neurotrophic factors, suggesting the beneficial properties of the FIFs for axonal regeneration. Thus, these results argue for the continual use of autologous transplantation as a novel and promising cell therapy for the treatment of spinal cord injury.