Isolation and characterization of an age-related antigen present on senescent human red blood cells.
Isolation and characterization of an age-related antigen present on senescent human red blood cells.
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衰老人红细胞上存在的年龄相关抗原的分离和表征。
DOI：
10.1182/blood.v58.2.341.341
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发表时间：
1981
期刊：
影响因子：
20.3
通讯作者：
RE Lovins
中科院分区：
文献类型：
--
作者：
EM Alderman;HH Fudenberg;RE Lovins
以固定化麦胚凝集素和固定化抗人免疫球蛋白（IG）为免疫吸附剂，从比密度大于1.110的人红细胞（RBC）亚群中，用纯化的RBC膜糖蛋白制备物进行亲和层析，分离出自体膜结合IgG。当在离液剂的存在下通过Sephadex G-200上的色谱进一步分离时，由此获得的含Ig的群体产生四个峰（Ia、Ib、II和III）。双重免疫扩散显示前三个峰中存在IG（峰Ia中的IgM、Ib中的伊加和II中的IgG），但峰III中不存在。在免疫扩散试验中，峰III被含Ig峰（Ia、Ib和II）沉淀，表明负责自体IG与衰老人RBC结合的抗原膜决定簇包含在该峰（III）中。峰Ia，Ib和II沉淀纯化的脱唾液酸血型糖蛋白;峰III与针对脱唾液酸血型糖蛋白的纯化自身抗体反应。这些结果表明，存在于衰老的人RBC上的年龄相关抗原决定簇通过RBC膜的主要唾液酸糖蛋白组分的去唾液酸化而暴露。
Autologous membrane-bound IgG was isolated from a subpopulation of human red blood cells (RBC) with specific density greater than 1.110, by affinity chromatography of purified RBC membrane glycoprotein preparations using immobilized wheat germ agglutinin and immobilized anti-human immunoglobulin (Ig) as immunoabsorbents. The Ig-containing population thus obtained, when further separated by chromatography on Sephadex G-200 in the presence of chaotropic agents, yielded four peaks (Ia, Ib, II, and III). Double immunodiffusion revealed the presence of Ig in the first three peaks (IgM in peak Ia, IgA in Ib, and IgG in II) but not in peak III. Peak III was precipitated by the Ig-containing peaks (Ia, Ib, and II) in immunodiffusion assays, suggesting that the antigenic membrane determinants responsible for the binding of autologous Ig to senescent human RBC were contained in this peak (III). Peaks Ia, Ib and II precipitate purified asialoglycophorin; peak III was reactive with purified autoantibodies directed against asialoglycophorin. These results suggest that an age-related antigenic determinant(s) present on senescent human RBC is exposed by desialylation of the major sialoglycoprotein component of the RBC membrane.