Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
Poly(lactide-co-glycolide)/silver nanoparticles: Synthesis, characterization, antimicrobial activity, cytotoxicity assessment and ROS-inducing potential
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DOI：
10.1016/j.polymer.2012.04.057
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发表时间：
2012-06
期刊：
影响因子：
4.6
通讯作者：
M. Stevanović;S. Škapin;Ines Bračko;M. Milenković;Jana Petković;M. Filipič;D. Uskoković
中科院分区：
文献类型：
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作者：
M. Stevanović;S. Škapin;Ines Bračko;M. Milenković;Jana Petković;M. Filipič;D. Uskoković
以聚α，γ，l-谷氨酸（PGA）为包覆剂，采用改进的化学还原法制备了银纳米粒子（AgNps）。这些Ag/PGA纳米颗粒（AgNpPGA）在长时间内高度稳定，没有沉淀的迹象。除了获得稳定的AgNpPGA外，另一个目的是检查它们在聚（L-丙交酯-共-乙交酯）（PLGA）聚合物基质中的包封。聚合物-AgNps在生物医学应用中的当前兴趣是因为多功能系统必须在目标接触时具有抗微生物活性，而不释放有毒的杀生物剂。这些PLGA/AgNpPGA的合成使用具有溶剂/非溶剂系统的物理化学方法。这些PLGA/AgNpPGA的降解和他们的AgNPs的释放速率在生理溶液中超过三个月的研究。采用微量肉汤稀释法对6株美国典型培养物保藏中心（ATCC）的实验室对照菌株和1株临床分离的耐甲氧西林金黄色葡萄球菌菌株的抗菌活性进行了研究，结果表明PLGA/AgNpPGA具有上级和扩展的抗菌活性。3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四氮唑溴化物试验表明这些PLGA/AgNpPGA具有良好的生物相容性。使用荧光探针通过荧光定量法测量细胞内活性氧的形成，这表明这些PLGA/AgNpPGA不是这种物质的诱导剂。采用紫外-可见光谱、X射线衍射、zeta电位、场发射扫描电子显微镜和透射电子显微镜对样品进行了表征。
Silver nanoparticles (AgNps) were prepared by modified chemical reduction with poly (α, γ, l-glutamic acid) (PGA) as capping agent. These Ag/PGA nanoparticles (AgNpPGAs) were highly stable over long periods of time without signs of precipitation. In addition to obtaining stable AgNpPGAs, a further aim was to examine their encapsulation in the poly(L-lactide-co-glycolide) (PLGA) polymer matrix. The current interest of polymer-AgNps in biomedical applications is because a versatile system must have antimicrobial activity upon target contact, without the release of toxic biocides. The synthesis of these PLGA/AgNpPGAs used physicochemical methods with solvent/non-solvent systems. Degradation of these PLGA/AgNpPGAs and the release rate of their AgNPs were studied in physiological solution over three months. The antimicrobial activity of the samples was investigated towards six laboratory control strains from the American Type Culture Collection (ATCC) and one clinical isolate methicillin-resistant Staphylococcus aureus strain by the broth microdilution method and the results showed superior and extended activity of PLGA/AgNpPGAs. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated good biocompatibility of these PLGA/AgNpPGAs. The formation of intracellular reactive oxygen species was measured spectrophotometrically using a fluorescent probe, which showed that these PLGA/AgNpPGAs are not inducers of such species. The samples were characterized by UV–VIS spectrometry, X-ray diffraction, zeta potential measurements, field-emission scanning electron microscopy, and transmission electron microscopy.