Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI
Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI
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DOI：
10.1182/blood-2004-02-0655
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发表时间：
2004-08-15
期刊：
影响因子：
20.3
通讯作者：
Frank, JA
中科院分区：
文献类型：
--
作者：
Arbab, AS;Yocum, GT;Frank, JA
最近，已经有几个报告使用各种超顺磁性氧化铁（SPIO）纳米粒子标记哺乳动物细胞，通过磁共振成像（MRI）监测其在体内的时间和空间迁移。本研究的目的是评价使用2种市售食品药品监督管理局（FDA）批准的试剂标记细胞的效率和毒性，ferumoxides是一种用作MRI造影剂的葡聚糖包被SPIO混悬液，硫酸鱼精蛋白通常用于逆转肝素抗凝，但也用作离体阳离子转染剂。用铁氧化物-硫酸鱼精蛋白复合物（FE-Pro）标记人骨髓间充质干细胞（MSCs）和造血（CD 34（+））干细胞以及其他哺乳动物细胞后，测定细胞毒性、功能能力和定量细胞铁掺入。与未标记的细胞相比，FE-Pro标记的细胞没有表现出短期或长期毒性，干细胞分化能力的变化或表型的变化。观察到FE-Pro的有效标记，每个细胞的铁含量在CD 34（+）细胞的2.01 +/- 0.1 pg和MSC的10.94 +/- 1.86 pg之间变化，100%的细胞被标记。使用这些试剂的细胞标记应有助于将该方法转化为临床试验，以通过MRI评估输注或移植细胞的运输。
Recently, there have been several reports using various superparamagnetic iron oxide (SPIO) nanoparticles to label mammalian cells for monitoring their temporal and spatial migration in vivo by magnetic resonance imaging (MRI). The purpose of this study was to evaluate the efficiency and toxicity of labeling cells using 2 commercially available Food and Drug Administration (FDA)-approved agents, ferumoxides, a suspension of dextran-coated SPIO used as an MRI contrast agent, and protamine sulfate, conventionally used to reverse heparin anticoagulation but also used ex vivo as a cationic transfection agent. After labeling of human mesenchymal stem cells (MSCs) and hematopoietic (CD34(+)) stem cells and other mammalian cells with ferumoxides-protamine sulfate complexes (FE-Pro), cellular toxicity, functional capacity, and quantitative cellular iron incorporation were determined. FE-Pro-labeled cells demonstrated no short-or long-term toxicity, changes in differentiation capacity of the stem cells, or changes in phenotype when compared with unlabeled cells. Efficient labeling with FE-Pro was observed with iron content per cell varying between 2.01 +/- 0.1 pg for CD34(+) cells and 10.94 +/- 1.86 pg for MSCs with 100% of cells labeled. Cell labeling using these agents should facilitate the translation of this method to clinical trials for evaluation of trafficking of infused or transplanted cells by MRI.