Cloning and functional expression of invertebrate connexins from Halocynthia pyriformis.
Cloning and functional expression of invertebrate connexins from Halocynthia pyriformis.
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梨状盐藻无脊椎动物连接蛋白的克隆和功能表达。
DOI：
10.1016/j.febslet.2004.09.071
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Brink,PeterR
中科院分区：
文献类型：
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作者：
White,ThomasW;Wang,Huan;Mui,Rickie;Litteral,Jennifer;Brink,PeterR
与许多其他离子通道不同，不相关的基因家族编码不同动物门中的缝隙连接。连接蛋白和pAnnexin基因在后口动物中被发现，而原口动物物种使用内联蛋白基因。连接蛋白通常被描述为脊椎动物的基因，尽管存在无脊椎动物后口动物。我们克隆了一种无脊椎动物梨形梭梭的连接蛋白序列。无脊椎动物连接蛋白与人类连接蛋白有25%-40%的序列同源性，含有6个不变的半胱氨酸残基的胞外结构域，编码被内含子打断的区域，并在体外形成功能通道。这些数据表明，在脊椎动物进化之前，就存在基于连接蛋白的缝隙连接通道。
Unlike many other ion channels, unrelated gene families encode gap junctions in different animal phyla. Connexin and pannexin genes are found in deuterostomes, while protostomal species use innexin genes. Connexins are often described as vertebrate genes, despite the existence of invertebrate deuterostomes. We have cloned connexin sequences from an invertebrate chordate, Halocynthia pyriformis. Invertebrate connexins shared 25–40% sequence identity with human connexins, had extracellular domains containing six invariant cysteine residues, coding regions that were interrupted by introns, and formed functional channels in vitro. These data show that gap junction channels based on connexins are present in animals that predate vertebrate evolution.