Ephus: multipurpose data acquisition software for neuroscience experiments.
Ephus: multipurpose data acquisition software for neuroscience experiments.
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DOI：
10.3389/fncir.2010.00100
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发表时间：
2010
影响因子：
3.5
通讯作者：
Shepherd GM
中科院分区：
文献类型：
--
作者：
Suter BA;O'Connor T;Iyer V;Petreanu LT;Hooks BM;Kiritani T;Svoboda K;Shepherd GM
神经科学实验中的生理测量通常涉及复杂的刺激范例和多个数据通道。Ephus()是为通用数据采集和仪器控制而设计的开源软件包。Ephus作为一组模块化程序运行，包括用于典型电生理实验中的单电极或多电极标准全细胞记录的Ephys程序，以及用于突触电路映射实验的MAPPER程序，该实验涉及基于谷氨酸去除或通道视紫红质-2兴奋的激光扫描光刺激。自定义用户功能允许用户在多个层面上进行扩展，包括在线分析和闭环实验，其中可以根据最近获得的数据更改实验参数，例如在活体行为实验期间。Ephus与各种数据采集和成像硬件兼容。本文介绍了Ephus的主要特点和模块，以及它们在典型实验应用中的应用。
Physiological measurements in neuroscience experiments often involve complex stimulus paradigms and multiple data channels. Ephus () is an open-source software package designed for general-purpose data acquisition and instrument control. Ephus operates as a collection of modular programs, including an ephys program for standard whole-cell recording with single or multiple electrodes in typical electrophysiological experiments, and a mapper program for synaptic circuit mapping experiments involving laser scanning photostimulation based on glutamate uncaging or channelrhodopsin-2 excitation. Custom user functions allow user-extensibility at multiple levels, including on-line analysis and closed-loop experiments, where experimental parameters can be changed based on recently acquired data, such as during in vivo behavioral experiments. Ephus is compatible with a variety of data acquisition and imaging hardware. This paper describes the main features and modules of Ephus and their use in representative experimental applications.