Genetic instability in lung cancer: concurrent analysis of chromosomal, mini- and microsatellite instability and loss of heterozygosity.
Genetic instability in lung cancer: concurrent analysis of chromosomal, mini- and microsatellite instability and loss of heterozygosity.
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DOI：
10.1038/sj.bjc.6603121
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发表时间：
2006-05-22
影响因子：
8.8
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为了探讨什么样的遗传不稳定性在肺癌发生中起重要作用，我们分析了 55 例肺癌的微卫星不稳定性、小卫星不稳定性、杂合性丢失 (LOH) 和染色体不稳定性，其中包括 10 例鳞状细胞癌、5 例大细胞癌和 3 例小细胞癌以及 37 例腺癌。小卫星不稳定性的分析其机制与微卫星不稳定性不同，此前尚未见报道。仅在 1 例中检测到小卫星不稳定性（1/55，1.8%），且微卫星不稳定性发生频率较低，仅在 3 例中检测到（3/55，5.5%）。相比之下，在 27 例 (49.1%) 病例中观察到至少在一个位点出现 LOH。在腺癌中，低分化癌中 LOH 的发生率高于高分化癌。对于染色体不稳定性，在腺癌中观察到分化等级和不稳定性之间存在类似的相关性。不稳定性在大细胞癌和小细胞癌中比在腺癌中更常见。我们的分析表明，染色体不稳定性和 LOH，而不是小卫星和微卫星不稳定性，在肺癌的发展中发挥着重要作用。
To investigate what kind of genetic instability plays important roles in lung carcinogenesis, we analyzed micro- and minisatellite instability, loss of heterozygosity (LOH) and chromosome instability in 55 cases of lung cancer, including, 10 squamous cell, 5 large cell, and 3 small cell carcinomas, and 37 adenocarcinomas. Analysis of minisatellite instability, the mechanism of which is different from microsatellite instability, has not been reported previously. Minisatellite instability was detected in only one case (1/55, 1.8%), and the frequency of microsatellite instability was low, being found only in three cases (3/55, 5.5%). In contrast, LOH, for at least in one locus, was observed in 27 cases (49.1%). In adenocarcinomas, the frequency of LOH was higher in poorly differentiated compared to more differentiated carcinomas. For chromosome instability, a similar correlation between differentiation grade and instability was observed in adenocarcinomas. And instability was more common in large cell and small cell carcinomas than in adenocarcinomas. Our analysis showed that chromosome instability and LOH, rather than mini- and microsatellite instability, play significant roles in the development of lung cancer.