Differential accumulation of high and low molecular weight heat shock proteins in Basmati rice (Oryza sativa L.) cultivars
Differential accumulation of high and low molecular weight heat shock proteins in Basmati rice (Oryza sativa L.) cultivars
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DOI：
10.1007/s10722-009-9451-1
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Hameed, A.
中科院分区：
文献类型：
--
作者：
Iqbal, N.;Farooq, S.;Hameed, A.
研究了克什米尔巴斯马蒂植物中各种热激蛋白（HSPs）的积累及其与内建耐寒性的关系。五个Basmati水稻品种（Oryza sativa），Basmati-370、Basmati-Pak、Basmati-198、Basmati-385和Kashmir Basmati被给予45和50 A ℃的温度冲击。在预热至45和50 A ℃和85%相对湿度的培养箱中进行16 h的温度冲击。提取蛋白质并在10%丙烯酰胺凝胶上分离，厚度为1 mm，并观察蛋白质级分。40 kDa的热休克蛋白的积累，观察到在所有的品种，20 kDa的热休克蛋白，特别是在克什米尔Basmati。少量的高分子量热休克蛋白在未经处理（对照）的克什米尔Basmati植物，它大大增加热休克后。20 kDa HSP仅在热处理的克什米尔Basmati中表达。详细描述了供试品种中热激蛋白表达的差异。
The accumulation of various heat shock proteins (HSPs) and their relationship with the inbuilt cold tolerance observed in Kashmir Basmati was studied. Five Basmati rice verities (Oryza sativa), Basmati-370, Basmati-Pak, Basmati-198, Basmati-385 and Kashmir Basmati were given temperature shock of 45 and 50A degrees C. Temperature shocks were given for 16 h in incubator preheated to 45 and 50A degrees C and 85% relative humidity. Proteins were extracted and separated on 10% acrylamide gels with 1 mm thickness and visualized for protein fractions. Accumulation of 40 kDa HSPs were observed in all the cultivars, and 20 kDa HSPs specifically in Kashmir Basmati. Small amounts of high molecular weight HSPs were observed in un-treated (control) plants of Kashmir Basmati, and it increased considerably after heat shock. The 20 kDa HSP was only expressed in heat-treated Kashmir Basmati. Differences in the expression of heat shock proteins in the tested varieties have been described in detail.