Cellular immunity in the insect Galleria mellonella against insect non-parasitic nematodes
Cellular immunity in the insect Galleria mellonella against insect non-parasitic nematodes
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DOI：
10.1017/s003118201800210x
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发表时间：
2018-12
期刊：
影响因子：
2.4
通讯作者：
Masaya Ono;T. Yoshiga
中科院分区：
文献类型：
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作者：
Masaya Ono;T. Yoshiga
对微生物感染的免疫是众所周知的；然而，关于对寄生多细胞生物免疫的信息仍然缺乏。为了了解先天宿主对线虫的细胞免疫，我们比较了大蜡蛾（Galleria mellonella）幼虫对非寄生、取食细菌的线虫秀丽隐杆线虫和致病性噬菌体异habditis的细胞反应。将完整的秀丽隐杆线虫的1龄或4龄幼虫注射到成熟线虫的幼虫体内，与致病线虫一样，大多数线虫是活的，并且不受周围昆虫血细胞反应的限制（包封），而在接种24 h后，两种热致死线虫的大多数线虫都被严重包封。其他非寄生线虫也未被包膜。令人惊讶的是，秀丽隐杆线虫注射到昆虫血腔中，在活体昆虫中生长和繁殖，导致宿主昆虫死亡。我们的研究结果表明秀丽隐杆线虫具有一些基本的机制来逃避嗜黑线虫的免疫并在血腔中生长。
Abstract Immunity to microbial infections is well understood; however, information regarding the immunity to parasitic multicellular organisms remains lacking. To understand innate host cellular immunity to nematodes, we compared the cellular response of the greater wax moth (Galleria mellonella) larvae against the non-parasitic, bacterial-feeding nematode Caenorhabditis elegans and pathogenic nematode Heterorhabditis bacteriophora. When intact first-instar or dauer larvae of C. elegans were injected into a G. mellonella larva, most of the nematodes were alive and not confined by the surrounding reaction by insect haemocytes (encapsulation), similarly as the pathogenic nematode, whereas most of the heat-killed nematodes of both species were severely encapsulated by 24 h after inoculation. Other non-parasitic nematodes were also not encapsulated. Surprisingly, C. elegans injected into the insect haemocoel grew and propagated in the live insect, resulting in death of the host insect. Our results suggest that C. elegans has some basic mechanisms to evade immunity of G. mellonenlla and grow in the haemocoel.