Progestin stimulation of thymidine kinase in the human breast cancer cell line T47D.
Progestin stimulation of thymidine kinase in the human breast cancer cell line T47D.
复制标题
孕激素刺激人乳腺癌细胞系 T47D 中的胸苷激酶。
DOI：
10.1016/0925-4439(91)90056-f
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Bircher,JA
中科院分区：
文献类型：
--
作者：
Moore,MR;Hathaway,LD;Bircher,JA
本实验室此前曾报道孕激素可促进人乳腺癌细胞株T47D的生长。为了进一步探讨这种刺激，我们正在研究胸苷激酶(EC2.7.1.21)对孕激素的影响，这是一种已知参与生长调节的酶。这份报告介绍了我们的发现，孕激素以剂量反应的方式在生理孕激素浓度下刺激胸苷激酶活性。雌二醇-17β也有刺激作用，但睾酮、氢化可的松和醛固酮不起作用。抗孕激素RU486抑制孕激素的刺激，但它本身也有刺激作用。孕激素刺激在24小时达到最大值，然后随着时间的推移逐渐减弱。放线菌素D和放线菌亚胺的实验表明，胸苷激酶的刺激依赖于新的RNA和蛋白质合成。这些数据进一步阐明了孕激素对人类乳腺癌生长的刺激作用。据我们所知，这是首次报道孕激素刺激人乳腺癌细胞中的胸苷激酶。
Our laboratory has previously reported that progestins stimulate growth of the human breast cancer cell line T47D. In an attempt to probe further into this stimulation, we are investigating progestin effects of thymidine kinase (EC 2.7.1.21), an enzyme known to be involved in growth regulation. This report relates our finding that progestins stimulate thymidine kinase activity, at physiological progestin concentrations, in a dose-responsive manner. Estradiol-17β also stimulates, but testosterone, hydrocortisone and aldosterone do not. The antiprogestin RU486 inhibits progestin stimulation, but also stimulates on its own. Maximal by 24 h, the progestin stimulation then falls off with time. Experiments with actinomycin D and cycloheximide suggest that the thymidine kinase stimulation depends on new RNA and protein synthesis. These data shed further light on progestin stimulation of the growth of human breast cancer. To our knowledge, this is the first report of progestin stimulation of thymidine kinase in human breast cancer cells.