VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells
VEGF induces angiogenesis in a zebrafish embryo glioma model established by transplantation of human glioma cells
复制标题
VEGF诱导人神经胶质瘤细胞移植建立的斑马鱼胚胎神经胶质瘤模型中的血管生成
DOI：
10.3892/or.2012.1861
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发表时间：
2012-09-01
期刊：
影响因子：
4.2
通讯作者：
Peng, Ying
中科院分区：
文献类型：
--
作者：
Li, Dong;Li, Xiang-Pen;Peng, Ying
斑马鱼（Danio rerio）正在成为一种越来越受欢迎的脊椎动物癌症模型。本研究建立了斑马鱼胚胎胶质瘤异种移植模型，以进一步探讨肿瘤血管生成的分子机制。我们发现胶质瘤细胞系U87可以在斑马鱼胚胎中存活、增殖并诱导额外的SIV分支。此外，原位杂交和定量RT-PCR分析发现，移植的U87细胞可诱导异位斑马鱼血管内皮生长因子A（VEGF-A）及其受体VEGFR 2/KDR mRNA的表达，并使其表达水平增加，导致SIV分支增多。
Zebrafish (Danio rerio) is becoming an increasingly popular vertebrate cancer model. In this study, we established a xenotransplanted zebrafish embryo glioma model to further investigate the molecular mechanisms of tumor angiogenesis. We find that the glioma cell line U87 can survive, proliferate and induce additional SIV branches in zebrafish embryos. In addition, by the means of in situ hybridization and quantitive RT-PCR analyses we find that the transplanted U87 cells can induce the ectopic zebrafish vascular endothelial growth factor A (VEGF A) and its receptor VEGFR2/KDR mRNA expression and increase their expression levels, resulting in additional SIV branches.