Stress-induced alternative splicing provides a mechanism for the regulation of microRNA processing in Arabidopsis thaliana.
Stress-induced alternative splicing provides a mechanism for the regulation of microRNA processing in Arabidopsis thaliana.
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DOI：
10.1016/j.molcel.2012.08.032
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发表时间：
2012-11
期刊：
影响因子：
16
通讯作者：
K. Yan;Peng Liu;Changai Wu;Guo-dong Yang;Rui Xu;Qianhuan Guo;Jinguang Huang;C. Zheng
中科院分区：
文献类型：
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作者：
K. Yan;Peng Liu;Changai Wu;Guo-dong Yang;Rui Xu;Qianhuan Guo;Jinguang Huang;C. Zheng
MicroRNAs(MiRNAs)在活细胞中通过转录后降解或翻译抑制而成为一类基因表达的调节因子。越来越多的证据表明miRNAs与环境胁迫反应之间的重要关系，但对植物中的调控机制知之甚少。在这里，我们发现拟南芥miR400与其宿主基因(At1g32583)共转录，并被热处理下调。有趣的是，发生在MIR400所在内含子(306个碱基)上的选择性剪接(AS)事件是由热应激特异性诱导的。去掉了100hp的片段，保留了含有MIR400转录本的206hp的内含子。因此，胁迫诱导的AS事件导致miR400初级转录本的大量积累和低水平的成熟miR400。综上所述，这些结果为AS作为一种调节机制将miRNAs与植物中的环境胁迫联系起来提供了更直接的证据。
MicroRNAs (miRNAs) have emerged as a class of regulators of gene expression through posttranscriptional degradation or translational repression in living cells. Increasing evidence points to the important relationship between miRNAs and environmental stress responses, but the regulatory mechanisms in plants are poorly understood. Here, we found thatArabidopsis thalianaintronic miR400 was cotranscribed with its host gene (At1g32583) and downregulated by heat treatment. Intriguingly, an alternative splicing (AS) event that occurred in the intron (306 bp) whereMIR400was located was specifically induced by heat stress. A 100 bp fragment was excised, and the remaining 206 bp intron containingMIR400transcripts was retained in the host gene. The stress-induced AS event thus resulted in greater accumulation of miR400 primary transcripts and a low level of mature miR400. Together, these results provide the direct evidence that AS acts as a regulatory mechanism linking miRNAs and environmental stress in plants.