Changes in the cellular makeup of motor patterning circuits drive courtship song evolution in Drosophila.
Changes in the cellular makeup of motor patterning circuits drive courtship song evolution in Drosophila.
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运动模式电路的细胞组成的变化推动了果蝇求爱歌曲的进化。
DOI：
10.1101/2024.01.23.576861
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Ding,Yun
中科院分区：
文献类型：
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作者：
Ye,Dajia;Walsh,JustinT;Junker,IanP;Ding,Yun
基因和神经元的进化变化如何编码复杂运动行为中的物种变异目前尚不清楚。在这里，我们开发了遗传工具，可以对模型物种果蝇和密切相关的物种 D 之间的神经回路进行比较。 yakuba，它经历了谱系特有的正弦鸣叫的丧失，正弦鸣叫是果蝇雄性求偶鸣叫的两种主要类型之一。对整个系统发育中称为 TN1 的歌曲模式神经元的神经解剖学比较表明，正弦歌曲的丧失与 TN1 神经元和支持正弦电路连接的神经突数量的减少之间存在联系。光遗传学激活证实 TN1 神经元 inD. 黑马已经失去了驱动正弦歌的能力，尽管他们仍然保持着驱动歌翼姿势的能力。单细胞转录组比较表明D. yakuba 特别缺乏与 TN1A 神经元相对应的细胞类型，TN1 亚型对于正弦歌曲至关重要。遗传和发育操作揭示了性别决定基因doublesexinD的功能差异。 yakubato 通过促进细胞凋亡来减少 TN1 数量。我们的工作阐明了运动模式回路和细胞类型变化在行为进化中的贡献，并揭示了性别决定基因重新配置神经回路的细胞构成的进化不稳定性。
How evolutionary changes in genes and neurons encode species variation in complex motor behaviors is largely unknown. Here, we develop genetic tools that permit a neural circuit comparison between the model speciesDrosophila melanogasterand the closely related speciesD. yakuba, which has undergone a lineage-specific loss of sine song, one of the two major types of male courtship song inDrosophila. Neuroanatomical comparison of song-patterning neurons called TN1 across the phylogeny demonstrates a link between the loss of sine song and a reduction both in the number of TN1 neurons and the neurites supporting the sine circuit connectivity. Optogenetic activation confirms that TN1 neurons inD. yakubahave lost the ability to drive sine song, although they have maintained the ability to drive the singing wing posture. Single-cell transcriptomic comparison shows thatD. yakubaspecifically lacks a cell type corresponding to TN1A neurons, the TN1 subtype that is essential for sine song. Genetic and developmental manipulation reveals a functional divergence of the sex determination genedoublesexinD. yakubato reduce TN1 number by promoting apoptosis. Our work illustrates the contribution of motor patterning circuits and cell type changes in behavioral evolution and uncovers the evolutionary lability of sex determination genes to reconfigure the cellular makeup of neural circuits.