The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling
The ZAR1 resistosome is a calcium-permeable channel triggering plant immune signaling
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ZAR1 抗性体是触发植物免疫信号的钙渗透通道
DOI：
10.1016/j.cell.2021.05.003
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发表时间：
2021-06-24
期刊：
影响因子：
64.5
通讯作者：
Zhou, Jian-Min
中科院分区：
文献类型：
--
作者：
Bi, Guozhi;Su, Min;Zhou, Jian-Min
核苷酸结合、富含亮氨酸的重复序列受体（NLRs）是植物和动物的主要免疫受体。拟南芥NLR蛋白ZAR1激活后，在体外形成五聚体抵抗体，引发植物免疫应答和细胞死亡。在本研究中，我们利用单分子成像技术表明，活化的ZAR1蛋白可以在质膜中形成五聚体复合物，ZAR1抵抗体在非洲蟾卵母细胞中显示离子通道活性，其方式依赖于位于通道孔中的保守酸性残基Glu11。预组装的ZAR1抗性小体很容易被整合到平面脂质双层中，并表现出钙渗透阳离子选择通道活性。此外，我们发现植物细胞中ZAR1的激活导致Glu11依赖的Ca2+内流，亚细胞结构的扰动，活性氧的产生和细胞死亡。因此，结果支持ZAR1抵抗体作为钙渗透阳离子通道触发免疫和细胞死亡。
Nucleotide-binding, leucine-rich repeat receptors (NLRs) are major immune receptors in plants and animals. Upon activation, the Arabidopsis NLR protein ZAR1 forms a pentameric resistosome in vitro and triggers immune responses and cell death in plants. In this study, we employed single-molecule imaging to show that the activated ZAR1 protein can form pentameric complexes in the plasma membrane, The ZAR1 resistosome displayed ion channel activity in Xenopus oocytes in a manner dependent on a conserved acidic residue Glu11 situated in the channel pore. Pre-assembled ZAR1 resistosome was readily incorporated into planar lipid-bilayers and displayed calcium-permeable cation-selective channel activity. Furthermore, we show that activation of ZAR1 in the plant cell led to Glu11 -dependent Ca2+ influx, perturbation of subcellular structures, production of reactive oxygen species, and cell death. The results thus support that the ZAR1 resisto-some acts as a calcium-permeable cation channel to trigger immunity and cell death.