High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates
High-Speed and Scalable Whole-Brain Imaging in Rodents and Primates
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DOI：
10.1016/j.neuron.2017.05.017
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发表时间：
2017-06-21
期刊：
影响因子：
16.2
通讯作者：
Hashimoto, Hitoshi
中科院分区：
文献类型：
--
作者：
Seiriki, Kaoru;Kasai, Atsushi;Hashimoto, Hitoshi
全脑的亚细胞分辨率成像和随后的图像分析是了解解剖和功能脑网络的先决条件。在这里，我们开发了一种非常高速的连续切片成像系统，名为FAST(块脸连续显微断层成像)，它可以在与光片荧光显微镜相当的速度范围内获取整个小鼠大脑的高分辨率图像。FAST能够以足以分辨所有细胞及其亚细胞结构的分辨率对大脑进行完整的可视化。FAST以高空间分辨率对整个大脑的正常脑细胞和疾病模型脑细胞进行无偏见的量化组比较。此外，FAST可高度扩展到非人类灵长类动物大脑和人类死后脑组织，并可以可视化整个成年绒猴大脑中的神经元投射。因此，FAST为全球方法提供了新的机会，使人们能够更好地了解多种动物模型和人类疾病中的大脑系统。
Subcellular resolution imaging of the whole brain and subsequent image analysis are prerequisites for understanding anatomical and functional brain networks. Here, we have developed a very high-speed serial-sectioning imaging system named FAST (block-face serial microscopy tomography), which acquires high-resolution images of a whole mouse brain in a speed range comparable to that of light-sheet fluorescence microscopy. FAST enables complete visualization of the brain at a resolution sufficient to resolve all cells and their subcellular structures. FAST renders unbiased quantitative group comparisons of normal and disease model brain cells for the whole brain at a high spatial resolution. Furthermore, FAST is highly scalable to non-human primate brains and human postmortem brain tissues, and can visualize neuronal projections in a whole adult marmoset brain. Thus, FAST provides new opportunities for global approaches that will allow for a better understanding of brain systems in multiple animal models and in human diseases.