Comprehensive analysis of serum metabolites in gestational diabetes mellitus by UPLC/Q-TOF-MS
Comprehensive analysis of serum metabolites in gestational diabetes mellitus by UPLC/Q-TOF-MS
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DOI：
10.1007/s00216-015-9211-3
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发表时间：
2016-02-01
影响因子：
4.3
通讯作者：
Dong, Fang
中科院分区：
文献类型：
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作者：
Liu, Tianhu;Li, Jiaxun;Dong, Fang
妊娠期糖尿病（GDM）是指妊娠期间而不是孕期糖尿病的首次征兆或发作。近几十年来，越来越多的研究集中在GDM的病因和发病机制上，以进一步了解GDM的进展和恢复情况。利用基于超高效液相色谱四极杆飞行时间质谱（UPLC/Q-TOF-MS）的先进代谢组学平台，我们探讨了妊娠期糖尿病女性和健康对照女性在妊娠期间和妊娠后血清代谢物的变化。使用多变量分析发现了一些显着差异，包括偏最小二乘判别分析 (PLS-DA) 和正交 PLS-DA (OPLS-DA)。在多个数据库中进一步比较和验证失调的代谢物，以了解这些化合物如何作为潜在的生物标志物发挥作用。随后探索了与这些潜在生物标志物相关的代谢途径的分析。共鉴定出35种代谢物，在一定程度上促进了GDM的进展。鉴定出的生物标志物涉及一些重要的代谢途径，包括甘氨酸、丝氨酸和苏氨酸代谢；类固醇激素生物合成；酪氨酸代谢；甘油磷脂代谢；和脂肪酸代谢。上述代谢途径主要参与人体三大代谢循环，包括脂质代谢、碳水化合物代谢和氨基酸代谢。在这项初步研究中，有价值的综合分析使我们进一步了解GDM的病因和病理生理学，这可能有利于尽快进行快速、准确的诊断和合理的治疗，同时也有利于预防GDM及其相关的短期和长期并发症。
Gestational diabetes mellitus (GDM) refers to the first sign or onset of diabetes mellitus during pregnancy rather than progestation. In recent decades, more and more research has focused on the etiology and pathogenesis of GDM in order to further understand GDM progress and recovery. Using an advanced metabolomics platform based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS), we explored the changes in serum metabolites between women with GDM and healthy controls during and after pregnancy. Some significant differences were discovered using multivariate analysis including partial least-squares discriminant analysis (PLS-DA) and orthogonal PLS-DA (OPLS-DA). The dysregulated metabolites were further compared and verified in several databases to understand how these compounds might function as potential biomarkers. Analyses of the metabolic pathways associated with these potential biomarkers were subsequently explored. A total of 35 metabolites were identified, contributing to GDM progress to some extent. The identified biomarkers were involved in some important metabolic pathways including glycine, serine, and threonine metabolism; steroid hormone biosynthesis; tyrosine metabolism; glycerophospholipid metabolism; and fatty acid metabolism. The above mentioned metabolic pathways mainly participate in three major metabolic cycles in humans, including lipid metabolism, carbohydrate metabolism, and amino acid metabolism. In this pilot study, the valuable comprehensive analysis gave us further insight into the etiology and pathophysiology of GDM, which might benefit the feasibility of a rapid, accurate diagnosis and reasonable treatment as soon as possible but also prevent GDM and its related short- and long-term complications.