Foxn4 directly regulates tbx2b expression and atrioventricular canal formation
Foxn4 directly regulates tbx2b expression and atrioventricular canal formation
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DOI：
10.1101/gad.1629408
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发表时间：
2008-03-15
影响因子：
10.5
通讯作者：
Stainier, Didier Y. R.
中科院分区：
文献类型：
--
作者：
Chi, Neil C.;Shaw, Robin M.;Stainier, Didier Y. R.
心腔形成代表了一个重要的进化里程碑，它允许心脏在整个封闭的循环系统中接收（心房）和泵送（心室）血液。在这里，我们揭示了一种新的转录途径之间的foxn 4和tbx基因，促进这一进化事件。我们发现，斑马鱼基因slipjig，其中编码Foxn 4，调节房室（AV）通道的形成，以划分心脏。sli/foxn 4在AV管中表达，其编码产物与高度保守的tbx 2增强子结构域结合，该增强子结构域含有Foxn 4-和T-box-结合位点，这两者都是调节tbx 2b在AV管中表达所必需的。
Cardiac chamber formation represents an essential evolutionary milestone that allows for the heart to receive (atrium) and pump (ventricle) blood throughout a closed circulatory system. Here, we reveal a novel transcriptional pathway between foxn4 and tbx genes that facilitates this evolutionary event. We show that the zebrafish gene slipjig, which encodes Foxn4, regulates the formation of the atrioventricular (AV) canal to divide the heart. sli/foxn4 is expressed in the AV canal, and its encoded product binds to a highly conserved tbx2 enhancer domain that contains Foxn4- and T-box-binding sites, both necessary to regulate tbx2b expression in the AV canal.