Characterization and Analysis of the Temporal and Spatial Dynamic of Several Enteritis Modeling Methodologies.
Characterization and Analysis of the Temporal and Spatial Dynamic of Several Enteritis Modeling Methodologies.
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几种肠炎建模方法的时空动态的表征和分析。
DOI：
10.3389/fimmu.2021.727664
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发表时间：
2021
影响因子：
7.3
通讯作者：
Hua ZC
中科院分区：
文献类型：
--
作者：
Xu H;Cai F;Li P;Wang X;Yao Y;Chang X;Bi Z;Sun H;Zhuang H;Hua ZC
炎症性肠病（IBD），如克罗恩病和溃疡性结肠炎，是一种涉及遗传、免疫和微生物因素的复杂疾病。目前已经开发了多种IBD动物模型来研究人类IBD的发病机制，但还没有一种模型能够完全代表IBD的复杂性。本研究分别通过口服3%DSS或腹腔注射抗CD 3抗体建立了两种急性肠炎模型，并通过喂食3个周期的1.5%DSS或3个月的高脂饮食建立了两种慢性肠炎模型，然后检查了临床参数、组织学变化以及细胞因子表达谱成功建立模型后的情况。结果表明，在3%DSS诱导的急性肠炎中，大肠损伤明显高于小肠，而在抗CD3抗体诱导的急性肠炎中，小肠损伤明显高于大肠损伤。另外，1.5%DSS引起的慢性肠炎损害主要集中在结直肠，而长期HFD引起的慢性肠炎损害更集中在小肠。因此，我们的工作提供了一个参考，选择合适的模型进行研究时，IBD的发病机制相关的因素或评估潜在的诊断和治疗药物的可能性。
Inflammatory bowel disease (IBD), such as Crohn’s disease and ulcerative colitis, is a complex disease involving genetic, immune, and microbiological factors. A variety of animal models of IBD have been developed to study the pathogenesis of human IBD, but there is no model that can fully represent the complexity of IBD. In this study, we established two acute enteritis models by oral 3% DSS or intraperitoneal injection of anti-CD3 antibody, and two chronic enteritis models by feeding 3 cycles of 1.5% DSS or 3 months of the high-fat diet, respectively, and then examined the clinical parameters, histological changes, and cytokine expression profiles after the successful establishment of the models. Our results indicated that in 3% DSS-induced acute enteritis, the colorectal injury was significantly higher than that of the small intestine, while in anti-CD3 antibody-induced acute enteritis, the small intestine injury was significantly higher than that of colorectal damage. Besides, in the 1.5% DSS-induced chronic enteritis, the damage was mainly concentrated in the colorectal, while the damage caused by long-term HFD-induced chronic enteritis was more focused on the small intestine. Therefore, our work provides a reference for selecting appropriate models when conducting research on factors related to the pathogenesis of IBD or evaluating the potential diagnosis and treatment possibilities of pharmaceuticals.
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影响因子：
32.4
作者：
Kontoyiannis, D;Pasparakis, M;Kollias, G
通讯作者：
Kollias, G
影响因子：
4.6
作者：
Gulhane M;Murray L;Lourie R;Tong H;Sheng YH;Wang R;Kang A;Schreiber V;Wong KY;Magor G;Denman S;Begun J;Florin TH;Perkins A;Cuív PÓ;McGuckin MA;Hasnain SZ
通讯作者：
Hasnain SZ
影响因子：
3.7
作者：
Kim KA;Gu W;Lee IA;Joh EH;Kim DH
通讯作者：
Kim DH
DOI：
10.1111/j.1440-1746.2010.06332.x
发表时间：
2010-11-01
影响因子：
4.1
作者：
Kim, In-Wha;Myung, Seung-Jae;Kim, Jin-Ho
通讯作者：
Kim, Jin-Ho
DOI：
10.1016/j.jnutbio.2011.07.002
发表时间：
2012-10
期刊：
The Journal of nutritional biochemistry
影响因子：
--
作者：
Liu Z;Brooks RS;Ciappio ED;Kim SJ;Crott JW;Bennett G;Greenberg AS;Mason JB
通讯作者：
Mason JB