A GLYCOPROTEIN ANTIGEN DETECTED WITH NEW MONOCLONAL-ANTIBODIES ON THE SURFACE OF HUMAN-LYMPHOCYTES INFECTED WITH HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I (HTLV-I)
A GLYCOPROTEIN ANTIGEN DETECTED WITH NEW MONOCLONAL-ANTIBODIES ON THE SURFACE OF HUMAN-LYMPHOCYTES INFECTED WITH HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I (HTLV-I)
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DOI：
10.1002/ijc.2910360506
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
HINUMA, Y
中科院分区：
文献类型：
--
作者：
TANAKA, Y;INOI, T;HINUMA, Y
我们制备了两种新的鼠单抗，分别命名为TARM-34(IgM)和Tag-34(IGGI)，它们与携带人T细胞白血病病毒I型(HTLV-I)的人淋巴细胞株的表面抗原发生反应。并与抗HTLV-I gp21单抗(TA-21，IGGI)、抗HTLV-Ip19单抗(GIN-14，IGGI)及成人T细胞白血病(ATL)人抗体进行了比较。间接膜免疫荧光试验表明，TARM-34、TAG-34和TA-21均能与HTLV-I阳性T、B细胞系和培养的HTLV-I感染成人外周血淋巴细胞发生特异性反应。放射免疫分析显示，ATL患者血清抗体干扰TA-21抗体与HTLV-I阳性T细胞系MT-2细胞的结合，但不干扰TARM-34和TAG-34抗体的结合。TARM-34和TAG-34均沉淀34kd的糖蛋白(GP34)，而TA-21则从~3H-葡萄糖胺标记的MT-2细胞裂解产物中沉淀gp21。TARM-34和TAG-34还从125I-或35S-半胱氨酸标记的MT-2和HUT 102细胞裂解产物中沉淀出34kd的蛋白。有趣的是，TARM-34和TAG-34也从来自ATL患者的其他HTLV-I阳性细胞(F-Taj细胞系)的裂解物中沉淀出35kd的蛋白。TA-21能从35S-半胱氨酸标记的HTLV-IMT-2病毒粒子裂解产物中沉淀出21kd的蛋白质，但TARM-34和TAG-34不能从裂解产物中沉淀任何蛋白质。在兔补体存在下，Tarm-34裂解携带HTLV的细胞。这些结果表明，TARM-34和TAG-34都识别HTLV-I感染细胞表面表达的糖蛋白抗原。
We have prepared two new mouse monoclonal antibodies (MAbs) named TARM-34 (IgM) and TAG-34 (IgGI), that react with surface antigens of lines of human lymphocytes bearing a human T-cell leukemia virus type-I (HTLV-I). The characters of these antibodies are compared with those of anti-HTLV-I gp21 MAb (TA-21, IgGI), anti-HTLV-I p19 MAb (GIN-14, IgGI) and human antibodies from patients with adult T-cell leukemia (ATL). An indirect membrane immunofluorescence assay showed that TARM-34, TAG-34 and TA-21 all reacted specifically with cell-surface antigens of HTLV-I-positive T- and B-cell lines and cultured peripheral blood lymphocyts from HTLV-I-infected adults. Radioimmunoassay showed that serum antibodies from the ATL patients interfered with the binding of TA-21 antibody to cells of the HTLV-I-positive T-cell line MT-2, but not with the bindings of TARM-34 and TAG-34 antibodies. TARM-34 and TAG-34 both precipitated a 34-kd glycoprotein (gP34), while TA-21 precipitated gp21 from a lysate of 3H-glucsamine-labelled MT-2 cells. TARM-34 and TAG-34 also precipitated the 34-kd protein from lysates of MT-2 and HUT 102 cells labelled with 125I- or 35S-cysteine. Interestingly, TARM-34 and TAG-34 also precipitated 35-kd protein from a lysate of other HTLV-I-positive cells (F-Taj cell line) derived from an ATL patient. TA-21 precipitated the 21-kd protein from the lysates of 35S-cysteine-labelled HTLV-IMT-2 virions, but TARM-34 and TAG-34 did not precipitate any protein from this lysate. TARM-34 lysed HTLV-bearing cells in the presence of rabbit complement. These results indicate that TARM-34 and TAG-34 both recognize a glycoprotein antigen that is expressed on the surface of HTLV-I-infected cells.