Proteomic analysis of the venom of Heterometrus longimanus (Asian black scorpion)
Proteomic analysis of the venom of Heterometrus longimanus (Asian black scorpion)
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DOI：
10.1002/pmic.200700948
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发表时间：
2008-03-01
期刊：
影响因子：
3.4
通讯作者：
Lipscombe, Richard
中科院分区：
文献类型：
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作者：
Bringans, Scott;Eriksen, Soren;Lipscombe, Richard
数百万年来，毒液已经进化成为生物活性肽和蛋白质的强效混合物。这些化合物对于制药工业的众多临床应用具有巨大价值。在这项研究中，采用了一种新颖的蛋白质组学-生物信息学方法，其中利用色谱法和凝胶电泳来分离 Heterometrus longimanus（亚洲黑蝎子）的毒液肽/蛋白质。通过串联质谱分析纯化的肽，从头测序，然后与 Swiss-Prot 蛋白质数据库中的已知肽进行同源性匹配。发现了许多潜在的生物活性肽匹配，并应用了一个简单的评分系统来推定为肽分配功能。作为对这种方法的验证，实验衍生的蛋白质组的功能组成与其他蝎子的蛋白质组相似，并且含有毒素、抗菌剂和离子通道抑制剂的有效混合物。
Venoms have evolved over millions of years into potent cocktails of bioactive peptides and proteins. These compounds can be of great value to the pharmaceutical industry for numerous clinical applications. In this study, a novel proteomic - bioinformatic approach was utilised, where chromatography followed by gel electrophoresis was utilised to separate the venom peptides/proteins of Heterometrus longimanus (Asian black scorpion). Purified peptides were analysed by tandem mass spectrometry, de novo sequenced and then homology matched against known peptides in the Swiss-Prot protein database. Numerous potentially biologically active peptide matches were discovered, and a simple scoring system applied to putatively assign functions to the peptides. As a validation of this approach, the functional composition of the experimentally derived proteome is similar to that of other scorpions, and contains a potent mix of toxins, antimicrobials and ionic channel inhibitors.