Cross-species complementation reveals conserved functions for EARLY FLOWERING 3 between monocots and dicots
Cross-species complementation reveals conserved functions for EARLY FLOWERING 3 between monocots and dicots
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DOI：
10.1002/pld3.18
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发表时间：
2017-10-01
期刊：
影响因子：
3
通讯作者：
Nusinow, Dmitri A.
中科院分区：
文献类型：
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作者：
Huang, He;Gehan, Malia A.;Nusinow, Dmitri A.
植物对环境的反应是由外部刺激和内部信号通路决定的。在模式植物拟南芥（Arabidopsis）和作物物种中，生物钟因素对于生长、开花和昼夜节律至关重要。然而，在拟南芥之外，人们对时钟基因产物的分子功能知之甚少。因此，我们试图比较拟南芥中关键时钟基因 EARLY FLOWERING 3 的二穗短柄草 (Brachypodium) 和狗尾草 (Setaria) 直向同源物的功能。为了鉴定狗尾草中的循环基因和假定的 ELF3 功能直系同源物，生成了昼夜节律 RNA-seq 数据集和在线查询工具 (Diel Explorer)，以探索狗尾草基因在昼夜节律条件下的表达谱。测试了来自拟南芥、短柄草和狗尾草的 ELF3 直系同源物的功能，以与拟南芥中的 elf3 突变互补。我们发现两种单子叶植物直系同源物都能够挽救下胚轴伸长、开花时间和心律失常的时钟表型。使用亲和纯化和质谱分析，我们的数据表明 BdELF3 和 SvELF3 可以在体内整合成与 AtELF3 类似的复合物。因此，我们发现，尽管分离了1.8亿年，BdELF3和SvELF3可以在分子和生理水平上功能性地补充ELF3的损失。
Plant responses to the environment are shaped by external stimuli and internal signaling pathways. In both the model plant Arabidopsis thaliana (Arabidopsis) and crop species, circadian clock factors are critical for growth, flowering, and circadian rhythms. Outside of Arabidopsis, however, little is known about the molecular function of clock gene products. Therefore, we sought to compare the function of Brachypodium distachyon (Brachypodium) and Setaria viridis (Setaria) orthologs of EARLY FLOWERING 3, a key clock gene in Arabidopsis. To identify both cycling genes and putative ELF3 functional orthologs in Setaria, a circadian RNA-seq dataset and online query tool (Diel Explorer) were generated to explore expression profiles of Setaria genes under circadian conditions. The function of ELF3 orthologs from Arabidopsis, Brachypodium, and Setaria was tested for complementation of an elf3 mutation in Arabidopsis. We find that both monocot orthologs were capable of rescuing hypocotyl elongation, flowering time, and arrhythmic clock phenotypes. Using affinity purification and mass spectrometry, our data indicate that BdELF3 and SvELF3 could be integrated into similar complexes in vivo as AtELF3. Thus, we find that, despite 180 million years of separation, BdELF3 and SvELF3 can functionally complement loss of ELF3 at the molecular and physiological level.