Translational and transcriptional analysis of Sulfolobus solfataricus P2 to provide insights into alcohol and ketone utilisation
Translational and transcriptional analysis of Sulfolobus solfataricus P2 to provide insights into alcohol and ketone utilisation
复制标题
DOI：
10.1002/pmic.200600746
复制
发表时间：
2007-02-01
期刊：
影响因子：
3.4
通讯作者：
Wright, Phillip C.
中科院分区：
文献类型：
--
作者：
Chong, Poh Kuan;Burja, Adam M.;Wright, Phillip C.
本研究探讨了硫磺硫化叶菌P2生物转化醇或酮的潜力。S.在0.4%w/v葡萄糖的存在下，在不同浓度（0.1- 0.8%w/v）的醇或酮（乙醇、异丙醇、正丙醇、丙酮、苯酚和己醇）中培养Solfataricus。因此，将这些醇或酮添加到生长培养基中对生物质产生具有抑制作用，从而与葡萄糖对照相比，滞后时间增加，比生长速率降低。在所有培养物中均观察到葡萄糖完全利用，但在实验培养物中观察到葡萄糖消耗速率较慢（平均值为14.9 mg/L/h，而对照组为18.9 mg/L/h）。另一方面，观察到溶剂利用不完全，最高溶剂消耗量约为丙酮培养物初始浓度的51%。S的翻译反应。然后使用相对和绝对定量的同量异位素标签（iTRAQ）技术研究了硫代硫酸盐对这些醇或酮的作用。与对照相比，鉴定和定量的大多数（> 80%）蛋白质在调节方面没有显示出可辨别的变化。这些结果，沿着从使用定量RT-PCR和代谢物分析的该分解代谢过程中涉及的关键基因的转录分析中获得的结果，证明了在S.太阳神
The potential of Sulfolobus solfataricus P2 for alcohol or ketone bioconversion was explored in this study. S. solfataricus was grown in different concentrations (0.1-0.8% w/v) of alcohols or ketones (ethanol, iso-propanol, n-propanol, acetone, phenol and hexanol) in the presence of 0.4% w/v glucose. Consequently, the addition of these alcohols or ketones into the growth media had an inhibitory effect on biomass production, whereby lag times increased and specific growth rates decreased when compared to a glucose control. Complete glucose utilisation was observed in all cultures, although slower rates of glucose consumption were observed in experimental cultures (average of 14.9 mg/L/h compared to 18.9 mg/L/h in the control). On the other hand, incomplete solvent utilisation was observed, with the highest solvent consumption being approximately 51% of the initial concentration in acetone cultures. Translational responses of S. solfataricus towards these alcohols or ketones were then investigated using the isobaric tags for relative and absolute quantitation (iTRAQ) technique. The majority (> 80%) of proteins identified and quantified showed no discernable changes in regulation compared to the control. These results, along with those obtained from transcriptional analysis of key genes involved within this catabolic process using quantitative RT-PCR and metabolite analysis, demonstrate successful alcohol or ketone conversion in S. solfataricus.