Photoactivation and inactivation of Arabidopsis cryptochrome 2.
Photoactivation and inactivation of Arabidopsis cryptochrome 2.
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拟南芥隐花色素 2 的光活化和失活
DOI：
10.1126/science.aaf9030
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发表时间：
2016-10-21
期刊：
影响因子：
--
通讯作者：
Lin C
中科院分区：
文献类型：
--
作者：
Wang Q;Zuo Z;Wang X;Gu L;Yoshizumi T;Yang Z;Yang L;Liu Q;Liu W;Han YJ;Kim JI;Liu B;Wohlschlegel JA;Matsui M;Oka Y;Lin C
关闭蓝光反应在植物中，蓝光被隐色素感知，一旦被激活，就会触发调节基因表达、昼夜节律和光形态形成的信号事件。Wang等人现在表明，在模式植物拟南芥中，激活的隐色素（活性时为二聚体或低聚物）的功能之一是激活BIC1蛋白（隐色素的蓝光抑制剂1）的产生（参见Fankhauser和Ulm的观点）。然后BIC1有利于单体化，从而使隐色素失活。这个反馈回路重置了系统，这样蓝光的反应既可以打开，也可以关闭。《科学》，本期第343页；另见第282页。反馈回路可防止植物对光的失控反应。隐色素是调节植物和动物发育和生物钟的蓝光受体。我们发现拟南芥隐色素2 （CRY2）经过蓝光依赖的同二聚化而具有生理活性。我们发现BIC1（蓝光隐色素抑制剂1）是植物隐色素的抑制剂，它与CRY2结合，抑制蓝光依赖性二聚化、光体形成、磷酸化、降解和CRY2的生理活性。我们假设在植物和其他进化谱系中，受调节的二聚化控制着活性隐色素的稳态。
Turning off the blue-light response In plants, blue light is perceived by cryptochromes, which, once activated, set off signaling events that regulate gene expression, circadian rhythms, and photomorphogenesis. Wang et al. now show that in the model plant Arabidopsis, one of the functions of activated cryptochromes, which are dimers or oligomers when active, is to activate production of the protein BIC1 (blue-light inhibitor of cryptochromes 1) (see the Perspective by Fankhauser and Ulm). BIC1 then favors monomerization and thus inactivation of the cryptochromes. This feedback loop resets the system so that blue-light responses can be turned off as well as turned on. Science, this issue p. 343; see also p. 282 A feedback loop ensures against a runaway response to light in plants. Cryptochromes are blue-light receptors that regulate development and the circadian clock in plants and animals. We found that Arabidopsis cryptochrome 2 (CRY2) undergoes blue light–dependent homodimerization to become physiologically active. We identified BIC1 (blue-light inhibitor of cryptochromes 1) as an inhibitor of plant cryptochromes that binds to CRY2 to suppress the blue light–dependent dimerization, photobody formation, phosphorylation, degradation, and physiological activities of CRY2. We hypothesize that regulated dimerization governs homeostasis of the active cryptochromes in plants and other evolutionary lineages.
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影响因子：
64.5
作者：
Pedmale UV;Huang SC;Zander M;Cole BJ;Hetzel J;Ljung K;Reis PAB;Sridevi P;Nito K;Nery JR;Ecker JR;Chory J
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56.9
作者：
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影响因子：
20.5
作者：
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